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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PA1-30829 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-PUMA Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- K562 or 3T3 cell lysate can be used as a positive control and a band at approximately 23 kDa can be detected. A lower band at approximately 16 kDa was detected in MOLT4 and U937 cells, which may represent the PUMA-beta form. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Caspase-3 triggers a TPCK-sensitive protease pathway leading to degradation of the BH3-only protein puma.
Hadji A, Clybouw C, Auffredou MT, Alexia C, Poalas K, Burlion A, Feraud O, Leca G, Vazquez A
Apoptosis : an international journal on programmed cell death 2010 Dec;15(12):1529-39
Apoptosis : an international journal on programmed cell death 2010 Dec;15(12):1529-39
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of PUMA in human (H) K562 and mouse (M) NIH-3T3 cell lysates using a PUMA polyclonal antibody (Product # PA1-30829) at a dilution of 2µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of PUMA in K562 cells using a PUMA polyclonal antibody (Product # PA1-30829) at a dilution of 1µg/mL.
