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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-34755 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PUMA alpha Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: K562, THP-1, HCT116 control 0hr, HCT116 treated 30uM cisplatin 24hr.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PUMA alpha Polyclonal Antibody (Product # PA5-34755). Sample (30 µg of whole cell lysate). Lane A: HCT116 cells with mock treatment for 0hr. Lane B: HCT116 cells with 30 µM cisplatin treatment for 24hr . 15% SDS PAGE. PUMA alpha Polyclonal Antibody (Product # PA5-34755) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PUMA delta/gamma Polyclonal Antibody detects PUMA protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 12 % SDS-PAGE, and blotted with PUMA delta/gamma Polyclonal Antibody PUMA alpha Polyclonal Antibody (Product # PA5-34755) diluted by 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human hepatoma, using PUMA (Product # PA5-34755) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PUMA alpha Polyclonal Antibody immunoprecipitates BCL2 binding component 3 protein in IP experiments. IP samples: HCT-116 whole cell extract treat with 30 µM cisplatin for 24 hr. A. Control with 4 µg of preimmune Rabbit IgG. B. Immunoprecipitation of BCL2 binding component 3 protein by 4 µg PUMA alpha Polyclonal Antibody (Product # PA5-34755). 15 % SDS-PAGE. The immunoprecipitated BCL2 binding component 3 protein was detected by PUMA alpha Polyclonal Antibody (Product # PA5-34755) diluted at 1:500.
