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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- MAB2755 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB2755, RRID:AB_10550389
- Product name
- BBC3 monoclonal antibody, clone 2A9G5
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against recombinant BBC3.
- Isotype
- IgG
- Antibody clone number
- 2A9G5
- Storage
- Store at 4°C for three months. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals.
PUMA, a novel proapoptotic gene, is induced by p53.
PUMA induces the rapid apoptosis of colorectal cancer cells.
Han J, Flemington C, Houghton AB, Gu Z, Zambetti GP, Lutz RJ, Zhu L, Chittenden T
Proceedings of the National Academy of Sciences of the United States of America 2001 Sep 25;98(20):11318-23
Proceedings of the National Academy of Sciences of the United States of America 2001 Sep 25;98(20):11318-23
PUMA, a novel proapoptotic gene, is induced by p53.
Nakano K, Vousden KH
Molecular cell 2001 Mar;7(3):683-94
Molecular cell 2001 Mar;7(3):683-94
PUMA induces the rapid apoptosis of colorectal cancer cells.
Yu J, Zhang L, Hwang PM, Kinzler KW, Vogelstein B
Molecular cell 2001 Mar;7(3):673-82
Molecular cell 2001 Mar;7(3):673-82
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Western blot analysis of BBC3 expression in K-562 cell lysate with BBC3 monoclonal antibody, clone 2A9G5 (Cat # MAB2755) at (A) 2.5 and (B) 5 ug/mL .
