Ab123948

antibody from Aladdin Scientific

Targeting: BBC3 JFY1, PUMA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

Ab123948

Provider

Aladdin Scientific

Product name

Recombinant PUMA Antibody

Antibody type

Recombinant

Description

Rabbit anti Human PUMA Antibody, Recombinant, could be used for WB, IHC, IF, ICC and so on.Application:WB: 1/500-1/1000IHC: 1/50-1/100ICC/IF: 1/50-1/200Protein FunctionEssential mediator of p53-dependent and p53-independent apoptosis.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

AA 177-193


Vial size

100_l,10_l,1ml,50_l

Concentration

0,3 mg/ml

Storage

Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Western blot

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Recombinant PUMA Antibody (Ab123948) - Western Blot All lanes: Recombinant PUMA Antibody (Ab123948) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 21 kDa Observed band size: 19, 25 kDa Exposure time: 8.0 s

Immunocytochemistry

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Recombinant PUMA Antibody (Ab123948) - IF/ICC Immunocytochemistry analysis of PUMA (green) in 293T using Recombinant PUMA Antibody (Ab123948), and cells were counterstained with DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Recombinant PUMA Antibody (Ab123948) - IHC Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Recombinant PUMA Antibody (Ab123948). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.