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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- NB100-56370 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-56370, RRID:AB_838746
- Product name
- Rabbit Polyclonal PUMA Antibody
- Antibody type
- Polyclonal
- Description
- Protein G purified. The amino acid sequence used as immunogen is 100% homologous in human (PUMA a and b) and 78% homologous in mouse and rat. Human PUMA a is a 193 amino acid protein with a predicted molecular weight of approximately 22 kD. Human PUMA b is a 131 amino acid protein with a predicted molecular weight of approximately 15 kD.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Effects of exosome-mediated delivery of myostatin propeptide on functional recovery of mdx mice.
Paradoxical Roles of Elongation Factor-2 Kinase in Stem Cell Survival.
SIRT1 activation by resveratrol ameliorates cisplatin-induced renal injury through deacetylation of p53.
Dissection of transcriptional and non-transcriptional p53 activities in the response to genotoxic stress.
PUMA expression is significantly reduced in human cutaneous melanomas.
Up-regulation of Bcl-2 homology 3 (BH3)-only proteins by E2F1 mediates apoptosis.
Ran N, Gao X, Dong X, Li J, Lin C, Geng M, Yin H
Biomaterials 2020 Apr;236:119826
Biomaterials 2020 Apr;236:119826
Paradoxical Roles of Elongation Factor-2 Kinase in Stem Cell Survival.
Liao Y, Chu HP, Hu Z, Merkin JJ, Chen J, Liu Z, Degenhardt K, White E, Ryazanov AG
The Journal of biological chemistry 2016 Sep 9;291(37):19545-57
The Journal of biological chemistry 2016 Sep 9;291(37):19545-57
SIRT1 activation by resveratrol ameliorates cisplatin-induced renal injury through deacetylation of p53.
Kim DH, Jung YJ, Lee JE, Lee AS, Kang KP, Lee S, Park SK, Han MK, Lee SY, Ramkumar KM, Sung MJ, Kim W
American journal of physiology. Renal physiology 2011 Aug;301(2):F427-35
American journal of physiology. Renal physiology 2011 Aug;301(2):F427-35
Dissection of transcriptional and non-transcriptional p53 activities in the response to genotoxic stress.
Speidel D, Helmbold H, Deppert W
Oncogene 2006 Feb 9;25(6):940-53
Oncogene 2006 Feb 9;25(6):940-53
PUMA expression is significantly reduced in human cutaneous melanomas.
Karst AM, Dai DL, Martinka M, Li G
Oncogene 2005 Feb 3;24(6):1111-6
Oncogene 2005 Feb 3;24(6):1111-6
Up-regulation of Bcl-2 homology 3 (BH3)-only proteins by E2F1 mediates apoptosis.
Hershko T, Ginsberg D
The Journal of biological chemistry 2004 Mar 5;279(10):8627-34
The Journal of biological chemistry 2004 Mar 5;279(10):8627-34
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PUMA Antibody [NB100-56370] - Analysis for Puma a (CT) using NB100-56370 at 1:500 against 30 ug/lane of A) Jurkat and B) NIH 3T3 whole cell lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PUMA Antibody [NB100-56370] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of a normal mouse lung using PUMA antibody at 1-200 dilution. The signal was developed using HRP-DAB detection and the sections were further counterstained using hematoxylin. The antibody generated a specific diffused cytoplasmic staining in the bronchiolar and alveolar epithelial cells. The representative section is showing alveolar area only.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PUMA Antibody [NB100-56370] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of a normal mouse lung using PUMA antibody at 1-200 dilution. The signal was developed using HRP-DAB detection and the sections were further counterstained using hematoxylin. The antibody generated a specific diffused cytoplasmic staining in the bronchiolar and alveolar epithelial cells.
