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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- MA5-17027 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATP Citrate Lyase Monoclonal Antibody (5F8D11)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-17027 targets ACLY in indirect ELISA, FACS, ICC, IHC, IF and WB applications and shows reactivity with Human, Mouse, Non-human primate, and Rat samples. The MA5-17027 immunogen is purified recombinant fragment of human ACLY (amino acids: 306-502 ) expressed in E. Coli. MA5-17027 detects ACLY which has a predicted molecular weight of approximately 125kDa.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 5F8D11
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ACLY using a ACLY monoclonal antibody (Product # MA5-17027) against a human ACLY recombinant protein.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ACLY using a ACLY monoclonal antibody (Product # MA5-17027) against a human ACLY recombinant protein.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ACLY using ACLY monoclonal antibody (Product # MA5-17027) in HeLa (1), NIH3T3 (2), C6 (3), COS-7 (4), and Raji (5) cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells using ACLY monoclonal antibody (Product # MA5-17027) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using ACLY monoclonal antibody (Product # MA5-17027) followed with DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using ACLY monoclonal antibody (Product # MA5-17027) followed with DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HeLa cells using ACLY monoclonal antibody (Product # MA5-17027) (green) and negative control (purple).
