711766

antibody from Invitrogen Antibodies

Targeting: ACLY ACL, ATPCL, CLATP

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

711766

Provider

Invitrogen Antibodies

Product name

ATP Citrate lyase Recombinant Polyclonal Antibody (8HCLC)

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Antibody clone number

8HCLC

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Knockdown of ATP Citrate lyase was achieved by transfecting A549 cells with ATP Citrate lyase specific validated siRNA (Silencer® select Product # s916). Western blot analysis was performed using Whole cell extracts from the ATP Citrate lyase knock down cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with Anti-ATP Citrate lyase Recombinant Rabbit Polyclonal Antibody (Product # 711766, 1-3 µg/mL) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). Loss of signal upon siRNA mediated knock down confirms that antibody is specific to ATP Citrate lyase.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on Whole cell extracts (30 µg lysate) of A549 (Lane 1), HeLa (Lane 2), NCI-H1975 (Lane 3), PC-3 (Lane 4), SK-OV-3 (Lane 5), A-431 (Lane 6), COS-7 (Lane 7), Jurkat (Lane 8), F9 (Lane 9) and tissue extracts of Mouse Lung (Lane 10). The blots were probed with Anti-ATP Citrate lyase Recombinant Rabbit Polyclonal Antibody (Product # 711766, 2.5 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 120 kDa band corresponding to ATP Citrate lyase was observed across the cell lines and tissues tested. Known quantity of protein samples were electrophoresed using Novex®NuPAGE®4-12% Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).