PA5-29497

ACL, ATPCL, CLATP

Antibody data

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Validation data

Product number: PA5-29497
Provider: Invitrogen Antibodies
Product name: Anti-ATP Citrate Lyase Polyclonal Antibody
Provider product page: Invitrogen Antibodies - PA5-29497
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: PA5-29497 targets ATP citrate lyase in IHC (P) and WB applications and shows reactivity with Human samples. The PA5-29497 immunogen is recombinant fragment corresponding to a region within amino acids 2 and 285 of Human ATP citrate lyase. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Provider	Type	Product Number
- No reagents -

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of ATP citrate lyase using 30 µg of A) 293T (B) A431 (C) Jurkat and D) Raji lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with an ATP citrate lyase polyclonal antibody (Product # PA5-29497) at a dilution of 1:5000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of ATP Citrate Lyase was achieved by transfecting A549 cells with ATP Citrate Lyase specific siRNAs (Silencer® select Product # s916). Western blot analysis (Fig. a) was performed using whole cell extracts from the ATP Citrate Lyase knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with ATP Citrate Lyase Polyclonal Antibody (Product # PA5-29497, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to ATP Citrate Lyase.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on whole cell extracts (30 µg lysate) of A549 (Lane 1), HeLa (Lane 2), PC-3 (Lane 3), SK-OV-3 (Lane 4), A-431 (Lane 5), Jurkat (Lane 6) and Raji (Lane 7). The blot was probed with Anti-ATP Citrate Lyase Polyclonal Antibody (Product # PA5-29497, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 120 kDa band corresponding to ATP Citrate Lyase was detected across the cell lines tested.