- PA5-29497 - Invitrogen Antibodies ACLY antibody

Xi Y, Harwood S, Wise LM, Purdy JG
Journal of virology 2019 Nov 1;93(21)

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of ATP citrate lyase using 30 µg of A) 293T (B) A431 (C) Jurkat and D) Raji lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with an ATP citrate lyase polyclonal antibody (Product # PA5-29497) at a dilution of 1:5000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis was performed on whole cell extracts (30 µg lysate) of A549 (Lane 1), HeLa (Lane 2), PC-3 (Lane 3), SK-OV-3 (Lane 4), A-431 (Lane 5), Jurkat (Lane 6) and Raji (Lane 7). The blot was probed with Anti-ATP Citrate Lyase Polyclonal Antibody (Product # PA5-29497, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 120 kDa band corresponding to ATP Citrate Lyase was detected across the cell lines tested.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of ATP Citrate Lyase was performed by separating 50 µg of various tissue extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a ATP Citrate Lyase Polyclonal Antibody (Product # PA5-29497) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using ATP Citrate Lyase Polyclonal Antibody (Product # PA5-29497). Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATP Citrate Lyase Polyclonal Antibody (Product # PA5-29497) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Knockdown of ATP Citrate Lyase was achieved by transfecting A549 cells with ATP Citrate Lyase specific siRNAs (Silencer® select Product # s916). Western blot analysis (Fig. a) was performed using whole cell extracts from the ATP Citrate Lyase knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with ATP Citrate Lyase Polyclonal Antibody (Product # PA5-29497, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to ATP Citrate Lyase.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunocytochemistry-Immunofluorescence analysis of ATP Citrate Lyase was performed in HeLa cells fixed in ice cold MeOH for 5 min. Green: ATP Citrate Lyase Polyclonal Antibody (Product # PA5 29497) diluted at 1:500. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: ATP Citrate Lyase Polyclonal Antibody detects ATP citrate lyase protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. ATP citrate lyase stained by ATP Citrate Lyase Polyclonal Antibody (Product # PA5-29497) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemical analysis of paraffin-embedded Hepatoma, using ATP citrate lyase (Product # PA5-29497) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

PA5-29497