NBP1-05210

antibody from Novus Biologicals

Targeting: NEFH NF-H, NFH

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-05210

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-05210, RRID:AB_1556326

Product name

Mouse Monoclonal NF-H Antibody

Antibody type

Monoclonal

Description

Affinity purified.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Vial size

0.1 ml

Concentration

1 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: NF-H Antibody (9B12) [NBP1-05210] - Strip blots of crude rat spinal cord extract stained with three different antibodies to phosphorylated NF-H, NB300-136 (lane 1), NBP1-05209 (Lane 2) and NBP1-05210 (lane 3). All three antibodies bind to a prominent band with an apparent SDS-PAGE molecular weight of 200kDa.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: NF-H Antibody (9B12) [NBP1-05210] - Analysis of different tissue lysates using mouse mAb to NF-H, dilution 1:10000 (Green): [1] protein standard, [2] rat spinal cord [3] mouse spinal cord, and [4] cow spinal cord. Strong band at about 200-220kDa corresponds to the major phosphorylated form of the NF-H subunit. Smaller proteolytic fragments of NF-H are also detected in some preparations.

Other assay

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry Free-Floating: NF-H Antibody (9B12) [NBP1-05210] - Analysis of a rat brain coronal section of the third ventricle stained with mouse monoclonal antibody to phosphorylated NF-H, dilution 1:5,000 (Green). Hoechst staining of nuclear DNA (Blue). Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24hrs, cut to 45uM, and free-floating sections were stained with above antibody. The antibody is a robust marker of the axons of neuronal cells.