OMA1-06117

antibody from Invitrogen Antibodies

Targeting: NEFH NF-H, NFH

Provider product page for OMA1-06117

Western blot

Immunohistochemistry

Other assay

Antibody data

Antibody Data
Antigen structure
References [1]
Comments [0]
Validations
Immunohistochemistry [1]
Other assay [3]

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Product number: OMA1-06117 - Provider product page
Provider: Invitrogen Antibodies
Product name: NF-H Monoclonal Antibody (RNF 405)
Antibody type: Monoclonal
Antigen: Other
Description: OMA1-06117 reacts exclusively with the phosphorylated form of the 200 kDa neurofilament from human, mouse, rat, canine, rabbit, monkey, bovine, ovine, hamster, chicken, guinea pig, and Xenopus laevis tissues. OMA1-06117 has been successfully used in Western blot and IHC (P) procedures. By Western blot, this antibody detects a single ~200 kDa protein representing phosphorylated neurofilament from rat brain homogenate. OMA1-06117 antigen is cytoskeletal preparation of calf brain tissue.
Reactivity: Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Guinea Pig, Hamster, Rabbit, Xenopus, Zebrafish
Host: Mouse
Isotype: IgG
Antibody clone number: RNF 405
Vial size: 100 µg
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

NEFH protein structure - OMA1-06117 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: FIGURE 5 Evaluation of neuronal outgrowth based on the 4-day culture of DRG explants obtained from rabbit lumbar spines. Immunofluorescent staining with neurofilament NF-200 antibody was used to identify neuronal outgrowth. Data from four rabbit donors were pooled for quantification. A,C, Representative images of outgrowth from DRG explant at 2% or 20% oxygen (scale bars equal 1000 mum). B,D, Magnified view of the regions of interest in A,C showing that the density of outgrowth at 20% oxygen was higher than at 2% oxygen (scale bars equal 200 mum). E, Image analysis showed that 2% oxygen induced significantly longer neuronal outgrowth than 20% oxygen (*** P < .001 by Mann-Whitney test, n = 230 and 1131 nerve fibers for 2% and 20% oxygen respectively). F, Frequency of neuronal outgrowth per DRG was significantly larger for 20% than for 2% oxygen (*** P < .001 by Mann-Whitney test, n = 22 and 20 explants for 2% and 20% oxygen, respectively)

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: FIGURE 6 Viability of a 4-day primary culture of isolated DRG neurons. The neurons were isolated from the DRGs of two rabbit spines. A,B, Representative images of DRG cells cultured at 2% and 20% oxygen for 4 days. Immunofluorescent staining with NF-200 antibody was used to distinguish neurons from other cell types and ethidium homodimer-1 was used to detect necrosis, which is shown by the arrows in the image. Scale bars equal 200 mum. C-F, When combining Hoechst staining with immunofluorescence, neuronal apoptosis could be observed as indicated by arrow heads. Scale bars equal 100 mum. G, Under 2% oxygen a significantly higher proportion of necrotic cells in each field was observed than in cultures kept at 20% oxygen. H Significantly lower proportion of apoptotic cells per field was found at 2% oxygen. I, No significant difference was identified for the proportion of viable neurons at 2% and 20% oxygen ( P = .769). J, The necrosis/apoptosis ratio per field was significantly higher for 2% than for 20% oxygen (*** P < .001 by Mann-Whitney test, n = 210 and 223 fields for 2% and 20% oxygen, respectively)

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: FIGURE 7 ""Sholl"" analysis of DRG neurons cultured at 2% and 20% oxygen for 2 days. A,B, Pattern images of DRG neurons (immunofluorescent staining with NF-200 antibody). C, Schematic showing the ""Sholl"" analysis of the outgrowth pattern. Circled samplings intersect with the neurite arbors providing information on how far the outgrowth can reach (represented as ending radius) and the number of branches (represented as number of intersections) at different distance from the soma (~30, 40-105, and 120-150 mum). D, Number and distribution of intersections at different distances from the soma. Note that the curve representing the mean number of intersections at 2% oxygen (blue line) is always above that at 20% oxygen (red line). E, Statistics showed that the intersection radius at 2% oxygen was significantly longer than at 20% oxygen. F, The sum of intersections of the whole cell was also significantly larger for 2% than for 20% oxygen. G, The initial branch number showed no significant difference between 2% and 20% oxygen. H, The magnitude of the difference in intersection number between 2% and 20% oxygen increased with distance from the soma. I Ramification index, which is the maximum number of intersections per sampling divided by the initial branch number, was significantly higher at 2% than at 20% oxygen (for box plot, * P < .05, ** P < .01, and *** P < .001 by Mann-Whitney test, n = 25 and 27 cells for 2% and 20% oxygen, respectively)