LS-C204553

antibody from LSBio

Targeting: NEFH NF-H, NFH

Western blot (Enhanced validation)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C204553

Provider

LSBio

Product name

NEFH / NF-H Antibody LS-C204553

Antibody type

Polyclonal

Description

Antiserum

Reactivity

Human, Bovine

Host

Rabbit

Storage

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Lane labeled CBB shows Coomassie Brilliant Blue stained crude extract of rat spinal cord, with the prominent major neurofilament subunit indicated. In rodents, NF-H runs at 200kDa, NF-M at 145kDa and NF-L at 68kDa. The Rab lane shows a blot probed with rabbit anti NF-H antibody NEFH / NF-H antibody. A prominent band at an apparent SDS-PAGE molecular weight of 200kDa corresponds to phosphorylated form of NF-H. The lane marked Rab shows a similar blot probed with our rabbit antibody to NF-H, CPCA-NF-H.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Shows a section of rat spinal cord stained with NEFH / NF-H antibody (green) and monoclonal antibody to ubiquitin C-terminal hydrolase 1 (UCHL1) clone MCA-BH7 (red). The neurofilament NF-H antibody binds primarily to phosphorylated axonal forms of NF-H, and so stains axons coursing between the large UCHL1 positive neurons. These large cells are motorneurons and UCHL1 protein is a major component of the perikarya and dendrites of these cells.