Antibody data
- Antibody Data
- Antigen structure
- References [40]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- 60331-1-Ig - Provider product page

- Provider
- Proteintech Group
- Product name
- NF-H/NF200 antibody
- Antibody type
- Monoclonal
- Description
- NF-H/NF200 antibody (Cat. #60331-1-Ig) is a mouse monoclonal antibody that shows reactivity with human, pig, mouse, rat and has been validated for the following applications: IF, IHC, WB, ELISA.
- Reactivity
- Human, Mouse, Rat, Porcine
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 1A3C7
- Vial size
- 20ul, 150ul
Submitted references Lactate drive M2 polarization via OXPHOS and Cebpb and accelerate peripheral nerve regeneration.
The effect of 807-nm light-emitting diode photobiomodulation on peripheral nerve injury.
Hydrogel with cell-cell adhesion cues enhances neural regeneration.
Screening Biomarkers for Nerve Injury Using Weighted Gene Co-Expression Network Analysis and Machine Learning.
Hydrogen-releasing electroactive nerve guidance conduits promote peripheral nerve regeneration by remodeling the microenvironment.
Injectable ion-coordinated double-network conductive hydrogel for spinal cord injury repair.
Tumor-Associated Sympathetic Nerves Promote the Progression of Epstein-Barr Virus-Positive Diffuse Large B-Cell Lymphoma.
Selenized neural stem cell-derived exosomes: A neotype therapeutic agent for traumatic injuries of the central nervous system.
Exercise Training Promotes Neural Remodeling and Vascular Regeneration in Cerebral Ischemic Rats Through the JNK/c-jun Signaling Pathway.
IGFBP2-Ceramide Pathway Mediates Divergent Myelin Breakdown in the PNS and CNS Following Injury.
Tetramethylpyrazine Promotes Functional Recovery after Spinal Cord Injury by Regulating 5-Methylcytosine RNA Modification via the NSUN2-XBP1 Axis.
Electrophysiological Evaluation of a Sciatic Nerve Degree III Injury Model in Rats.
Activation of NR2A-Wnt-TLR2 Signaling Axis in Satellite Glial Cells of the Dorsal Root Ganglion Contributes to Neuropathic Pain Induced by Nerve Injury in Diabetic Mice.
Refined qingkailing attenuates reactive astrocytes and glial scar formation after ischemia stroke via the EGFR/PLCγ pathway.
GABA(B) receptors regulate the neural stem cell potential of Pkd2l1(+) cerebrospinal fluid-contacting neurons via the PI3K/Akt signaling pathway.
VNS facilitates the neurological function recovery after ischemia/reperfusion injury by regulating the A1/A2 polarization of astrocytes through the NMU-NMUR2 pathway.
Acetylated chitosan scaffolds modulate the micro-environment and promote peripheral nerve regeneration through macrophage TLR2/4 pathway activation.
Engineered BMSC-derived exosomes deliver miR-17-5p inhibitor to restore sensory conduction via the IL-11/GP130/STAT3/GAP-43 axis in dorsal column lesion.
Glycine N-acyltransferase deficiency in sensory neurons suppresses osteoarthritis pain.
Lycium barbarum Glycopeptide Alleviates Neomycin-Induced Ototoxicity by Inhibiting Tryptophan Hydroxylase-Mediated Serotonin Biosynthesis.
Photo-Responsive H(2)S Composite System Regulates the Nerve Regeneration Microenvironment Through Multiple Pathways.
Exogenous NT-3 Promotes Phenotype Switch of Resident Macrophages and Improves Sciatic Nerve Injury through AMPK/NF-κB Signaling Pathway.
The role of JAK/STAT/SOCS3 signaling in rats with brain damage induced by early alcohol exposure after birth.
Brain Slice Derived Nerve Fibers Grow along Microcontact Prints and are Stimulated by Beta-Amyloid(42).
Pleiotropic effects of nitric oxide sustained-release system for peripheral nerve repair.
A Combination of Heavy Metals and Intracellular Pathway Modulators Induces Alzheimer Disease-like Pathologies in Organotypic Brain Slices.
Targeting Ferroptosis Promotes Functional Recovery by Mitigating White Matter Injury Following Acute Carbon Monoxide Poisoning.
Nicotine Decreases Nerve Regeneration and Pain Behaviors via PTEN and Downstream Inflammation-Related Pathway in Two Rat Nerve Injury Models.
Li-Mg-Si bioceramics provide a dynamic immuno-modulatory and repair-supportive microenvironment for peripheral nerve regeneration.
Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model.
Transcription factor ETS proto-oncogene 1 contributes to neuropathic pain by regulating histone deacetylase 1 in primary afferent neurons.
Optogenetic Stimulation of mPFC Alleviates White Matter Injury-Related Cognitive Decline after Chronic Ischemia through Adaptive Myelination.
An injectable and adaptable hydrogen sulfide delivery system for modulating neuroregenerative microenvironment.
Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia.
The Cytidine N-Acetyltransferase NAT10 Participates in Peripheral Nerve Injury-Induced Neuropathic Pain by Stabilizing SYT9 Expression in Primary Sensory Neurons.
Ursolic Acid Ameliorates Spinal Cord Injury in Mice by Regulating Gut Microbiota and Metabolic Changes.
Neutrophil Nanovesicle Protects against Experimental Autoimmune Encephalomyelitis through Enhancing Myelin Clearance by Microglia.
Apelin alleviated neuroinflammation and promoted endogenous neural stem cell proliferation and differentiation after spinal cord injury in rats.
Oriented nanofibrous P(MMD-co-LA)/Deferoxamine nerve scaffold facilitates peripheral nerve regeneration by regulating macrophage phenotype and revascularization.
Proanthocyanidin promotes functional recovery of spinal cord injury via inhibiting ferroptosis.
Li W, Wang X, Liu X, Wang L, Liang J, Cui Y, Liu Y, Zhou M, Yang Z, Song M, Yang Y, Zhang L, Zhang B
International immunopharmacology 2026 May 1;176:116474
International immunopharmacology 2026 May 1;176:116474
The effect of 807-nm light-emitting diode photobiomodulation on peripheral nerve injury.
Chang B, Quan Q, Liang X, Guan Y, Xiong X, Qiu H, Zeng J, Liu Y, Wang Y, Peng J, Gu Y
Photochemistry and photobiology 2026 Jan-Feb;102(1):207-219
Photochemistry and photobiology 2026 Jan-Feb;102(1):207-219
Hydrogel with cell-cell adhesion cues enhances neural regeneration.
Tang X, Zhang S, Liu M, Liang Y, Fan Y, Sun X, He C, Yang X, Zhu R, Jin X, Zhuang Q, Ge Y, Ling J, Yang Y
Nature communications 2026 Jan 19;17(1)
Nature communications 2026 Jan 19;17(1)
Screening Biomarkers for Nerve Injury Using Weighted Gene Co-Expression Network Analysis and Machine Learning.
Cao S, Yu C, Wang N, Xu J, Xu W
Brain and behavior 2026 Feb;16(2):e71279
Brain and behavior 2026 Feb;16(2):e71279
Hydrogen-releasing electroactive nerve guidance conduits promote peripheral nerve regeneration by remodeling the microenvironment.
Li Z, Su J, Dong X, Liang X, Huo Y, Yang J, Yan L, Yu A, Dai H
Biomaterials 2026 Apr;327:123752
Biomaterials 2026 Apr;327:123752
Injectable ion-coordinated double-network conductive hydrogel for spinal cord injury repair.
Yu H, Liu F, Hu Y, Wan W, Liu Q, Zhou S, Zhang L, Li L, Huang F
Frontiers in bioengineering and biotechnology 2025;13:1618680
Frontiers in bioengineering and biotechnology 2025;13:1618680
Tumor-Associated Sympathetic Nerves Promote the Progression of Epstein-Barr Virus-Positive Diffuse Large B-Cell Lymphoma.
Huang S, Lei D, Yang L, Bi A, Wang Y, Zhang P, Zhuang D, Liu H, Cai Q, Nie M, Xia Y
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Sep;12(33):e13580
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Sep;12(33):e13580
Selenized neural stem cell-derived exosomes: A neotype therapeutic agent for traumatic injuries of the central nervous system.
Wang W, Lu G, Guo P, Zhang H, Wang Y, Zheng D, Lyu C, Wang D, Li S, Li F, Zhao J, Qin M, Li W, Tan H, Ma G, Wei W
Cell reports. Medicine 2025 Sep 16;6(9):102319
Cell reports. Medicine 2025 Sep 16;6(9):102319
Exercise Training Promotes Neural Remodeling and Vascular Regeneration in Cerebral Ischemic Rats Through the JNK/c-jun Signaling Pathway.
Zhao Z, Chen X, Zhuang Z, Chen J, Zheng Z, Wu W, Wu Z, Lin Q, Chen M, Liu J, Liu N, Chen R, Chen H
Molecular neurobiology 2025 Oct;62(10):13103-13118
Molecular neurobiology 2025 Oct;62(10):13103-13118
IGFBP2-Ceramide Pathway Mediates Divergent Myelin Breakdown in the PNS and CNS Following Injury.
Ma S, Fu X, Lyu J, Guo W, Wang J, Li J, Ying Z
The Journal of neuroscience : the official journal of the Society for Neuroscience 2025 Oct 22;45(43)
The Journal of neuroscience : the official journal of the Society for Neuroscience 2025 Oct 22;45(43)
Tetramethylpyrazine Promotes Functional Recovery after Spinal Cord Injury by Regulating 5-Methylcytosine RNA Modification via the NSUN2-XBP1 Axis.
Xiao Z, Wang Z, Jiang H, Liu Y, Guo W
Molecular neurobiology 2025 Nov 19;63(1):74
Molecular neurobiology 2025 Nov 19;63(1):74
Electrophysiological Evaluation of a Sciatic Nerve Degree III Injury Model in Rats.
Chen L, Wang Z, Wang C, Ma N, Zhao W, Liu S, Lu J, Zhao B, Sun H, Che P, Dou N
Bio-protocol 2025 May 20;15(10):e5311
Bio-protocol 2025 May 20;15(10):e5311
Activation of NR2A-Wnt-TLR2 Signaling Axis in Satellite Glial Cells of the Dorsal Root Ganglion Contributes to Neuropathic Pain Induced by Nerve Injury in Diabetic Mice.
Zhang YY, Zhu DX, Wang MY, Yi YT, Feng YH, Zhou C, Li CJ, Liu F, Shen JF
Molecular neurobiology 2025 Jun;62(6):8013-8037
Molecular neurobiology 2025 Jun;62(6):8013-8037
Refined qingkailing attenuates reactive astrocytes and glial scar formation after ischemia stroke via the EGFR/PLCγ pathway.
Ren ZL, Zhang CX, Zheng YX, Chen CA, Dan-Chen, Lan X, Yan X, Liu Y, He YH, Cheng JL, Han JH, Wang QG, Wang XQ, Cheng FF, Li CX
Phytomedicine : international journal of phytotherapy and phytopharmacology 2025 Jul;142:156696
Phytomedicine : international journal of phytotherapy and phytopharmacology 2025 Jul;142:156696
GABA(B) receptors regulate the neural stem cell potential of Pkd2l1(+) cerebrospinal fluid-contacting neurons via the PI3K/Akt signaling pathway.
Cao L, Yan WH, Pi W, Zhang Y, Xiong YX, Yong VW, Xue M, Li Q, Zheng C, Yang L
Brain research bulletin 2025 Feb;221:111217
Brain research bulletin 2025 Feb;221:111217
VNS facilitates the neurological function recovery after ischemia/reperfusion injury by regulating the A1/A2 polarization of astrocytes through the NMU-NMUR2 pathway.
Jiang X, Yang W, Liu G, Tang H, Zhang R, Zhang L, Li C, Li S
Neurochemistry international 2025 Feb;183:105918
Neurochemistry international 2025 Feb;183:105918
Acetylated chitosan scaffolds modulate the micro-environment and promote peripheral nerve regeneration through macrophage TLR2/4 pathway activation.
Jiang Y, Li S, Chang M, Bian T, Wu W, Li T, Yang P, Yang Y
Materials today. Bio 2025 Dec;35:102376
Materials today. Bio 2025 Dec;35:102376
Engineered BMSC-derived exosomes deliver miR-17-5p inhibitor to restore sensory conduction via the IL-11/GP130/STAT3/GAP-43 axis in dorsal column lesion.
Wang Z, Zhang M, Wang F, Wang X, Song X, Wang X, Zhang L, Ren L, Jing X, Yang L, Wang T
International journal of biological macromolecules 2025 Dec;334(Pt 1):148958
International journal of biological macromolecules 2025 Dec;334(Pt 1):148958
Glycine N-acyltransferase deficiency in sensory neurons suppresses osteoarthritis pain.
Wang L, Luo J, Mao Z, Zhao W, Du S, Zhang Y, Cui Q, Liu H, Yang B, Zheng R, Guo S, Wang Y, Huo X, Zhang T, Zhang F, Li H
The journal of pain 2025 Aug;33:105408
The journal of pain 2025 Aug;33:105408
Lycium barbarum Glycopeptide Alleviates Neomycin-Induced Ototoxicity by Inhibiting Tryptophan Hydroxylase-Mediated Serotonin Biosynthesis.
Wu Y, Zhang L, Cao S, Zhang J, Li C, Shan Y, Liu Q, Yu Z, Fang Q, Zhang Y, Fu X, So KF, Chai R
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Aug;12(29):e2405850
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Aug;12(29):e2405850
Photo-Responsive H(2)S Composite System Regulates the Nerve Regeneration Microenvironment Through Multiple Pathways.
Huo Y, Tan X, Dong X, Liang X, Liu K, Zhang H, Li Z, Yang J, Pang Z, Yao Y, Yu A, Dai H
Advanced materials (Deerfield Beach, Fla.) 2025 Apr;37(13):e2413992
Advanced materials (Deerfield Beach, Fla.) 2025 Apr;37(13):e2413992
Exogenous NT-3 Promotes Phenotype Switch of Resident Macrophages and Improves Sciatic Nerve Injury through AMPK/NF-κB Signaling Pathway.
Sun X, Ni S, Zhou Q, Zou D
Neurochemical research 2024 Sep;49(9):2600-2614
Neurochemical research 2024 Sep;49(9):2600-2614
The role of JAK/STAT/SOCS3 signaling in rats with brain damage induced by early alcohol exposure after birth.
Yang C, Gui J, Huang D, Ding R, Liu J, Zhao W, Yang J, Han Z, Xie L, Yang X, Pan Y, Xie M, Cheng L, Song X, Jiang L
Pediatric discovery 2024 Jun;2(2):e64
Pediatric discovery 2024 Jun;2(2):e64
Brain Slice Derived Nerve Fibers Grow along Microcontact Prints and are Stimulated by Beta-Amyloid(42).
Steiner K, Humpel C
Frontiers in bioscience (Landmark edition) 2024 Jun 24;29(6):232
Frontiers in bioscience (Landmark edition) 2024 Jun 24;29(6):232
Pleiotropic effects of nitric oxide sustained-release system for peripheral nerve repair.
Huo Y, Cheng Y, Dong X, Cheng Q, Liang X, Duan P, Yu Y, Yan L, Qiu T, Pan Z, Dai H
Acta biomaterialia 2024 Jul 1;182:28-41
Acta biomaterialia 2024 Jul 1;182:28-41
A Combination of Heavy Metals and Intracellular Pathway Modulators Induces Alzheimer Disease-like Pathologies in Organotypic Brain Slices.
Korde DS, Humpel C
Biomolecules 2024 Jan 30;14(2)
Biomolecules 2024 Jan 30;14(2)
Targeting Ferroptosis Promotes Functional Recovery by Mitigating White Matter Injury Following Acute Carbon Monoxide Poisoning.
Wang S, Xiong B, Tian Y, Hu Q, Jiang X, Zhang J, Chen L, Wang R, Li M, Zhou X, Zhang T, Ge H, Yu A
Molecular neurobiology 2024 Feb;61(2):1157-1174
Molecular neurobiology 2024 Feb;61(2):1157-1174
Nicotine Decreases Nerve Regeneration and Pain Behaviors via PTEN and Downstream Inflammation-Related Pathway in Two Rat Nerve Injury Models.
Fang Y, Zhang T, Li L, Chen S, Wang L, Tang J, Liao Y
eNeuro 2023 Sep;10(9)
eNeuro 2023 Sep;10(9)
Li-Mg-Si bioceramics provide a dynamic immuno-modulatory and repair-supportive microenvironment for peripheral nerve regeneration.
Sun Y, Zhang H, Zhang Y, Liu Z, He D, Xu W, Li S, Zhang C, Zhang Z
Bioactive materials 2023 Oct;28:227-242
Bioactive materials 2023 Oct;28:227-242
Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model.
Hu M, Li T, Ma X, Liu S, Li C, Huang Z, Lin Y, Wu R, Wang S, Lu D, Lu T, Men X, Shen S, Huang H, Liu Y, Song K, Jian B, Jiang Y, Qiu W, Liu Q, Lu Z, Cai W
Nature communications 2023 Jul 4;14(1):3945
Nature communications 2023 Jul 4;14(1):3945
Transcription factor ETS proto-oncogene 1 contributes to neuropathic pain by regulating histone deacetylase 1 in primary afferent neurons.
Zheng HL, Sun SY, Jin T, Zhang M, Zeng Y, Liu Q, Yang K, Wei R, Pan Z, Lin F
Molecular pain 2023 Jan-Dec;19:17448069231152125
Molecular pain 2023 Jan-Dec;19:17448069231152125
Optogenetic Stimulation of mPFC Alleviates White Matter Injury-Related Cognitive Decline after Chronic Ischemia through Adaptive Myelination.
Deng S, Shu S, Zhai L, Xia S, Cao X, Li H, Bao X, Liu P, Xu Y
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2023 Feb;10(5):e2202976
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2023 Feb;10(5):e2202976
An injectable and adaptable hydrogen sulfide delivery system for modulating neuroregenerative microenvironment.
Dong X, Zhang H, Duan P, Liu K, Yu Y, Wei W, Wang W, Liu Y, Cheng Q, Liang X, Huo Y, Yan L, Yu A, Dai H
Science advances 2023 Dec 22;9(51):eadi1078
Science advances 2023 Dec 22;9(51):eadi1078
Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia.
Li T, Su X, Lu P, Kang X, Hu M, Li C, Wang S, Lu D, Shen S, Huang H, Liu Y, Deng X, Cai W, Wei L, Lu Z
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2023 Aug;10(22):e2206432
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2023 Aug;10(22):e2206432
The Cytidine N-Acetyltransferase NAT10 Participates in Peripheral Nerve Injury-Induced Neuropathic Pain by Stabilizing SYT9 Expression in Primary Sensory Neurons.
Zhang M, Yang K, Wang QH, Xie L, Liu Q, Wei R, Tao Y, Zheng HL, Lin N, Xu H, Yang L, Wang H, Zhang T, Xue Z, Cao JL, Pan Z
The Journal of neuroscience : the official journal of the Society for Neuroscience 2023 Apr 26;43(17):3009-3027
The Journal of neuroscience : the official journal of the Society for Neuroscience 2023 Apr 26;43(17):3009-3027
Ursolic Acid Ameliorates Spinal Cord Injury in Mice by Regulating Gut Microbiota and Metabolic Changes.
Rong ZJ, Cai HH, Wang H, Liu GH, Zhang ZW, Chen M, Huang YL
Frontiers in cellular neuroscience 2022;16:872935
Frontiers in cellular neuroscience 2022;16:872935
Neutrophil Nanovesicle Protects against Experimental Autoimmune Encephalomyelitis through Enhancing Myelin Clearance by Microglia.
Shen S, Cheng X, Zhou L, Zhao Y, Wang H, Zhang J, Sun X, Wang Y, Shu Y, Xu Y, Tao Y, Li M, Lu Z, Cai W, Nie G, Qiu W
ACS nano 2022 Nov 22;16(11):18886-18897
ACS nano 2022 Nov 22;16(11):18886-18897
Apelin alleviated neuroinflammation and promoted endogenous neural stem cell proliferation and differentiation after spinal cord injury in rats.
Liu Q, Zhou S, Wang X, Gu C, Guo Q, Li X, Zhang C, Zhang N, Zhang L, Huang F
Journal of neuroinflammation 2022 Jun 20;19(1):160
Journal of neuroinflammation 2022 Jun 20;19(1):160
Oriented nanofibrous P(MMD-co-LA)/Deferoxamine nerve scaffold facilitates peripheral nerve regeneration by regulating macrophage phenotype and revascularization.
Dong X, Wu P, Yan L, Liu K, Wei W, Cheng Q, Liang X, Chen Y, Dai H
Biomaterials 2022 Jan;280:121288
Biomaterials 2022 Jan;280:121288
Proanthocyanidin promotes functional recovery of spinal cord injury via inhibiting ferroptosis.
Zhou H, Yin C, Zhang Z, Tang H, Shen W, Zha X, Gao M, Sun J, Xu X, Chen Q
Journal of chemical neuroanatomy 2020 Sep;107:101807
Journal of chemical neuroanatomy 2020 Sep;107:101807
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image

- Experimental details
- pig brain tissue were subjected to SDS PAGE followed by western blot with 60331-1-Ig( NF200 Antibody) at dilution of 1:4000
- Validation comment
- WB
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image

- Experimental details
- Immunofluorescent analysis of SH-SY5Y cells using 60331-1-Ig( NF200 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
- Validation comment
- IF