LS-C204550

antibody from LSBio

Targeting: NEFH NF-H, NFH

Western blot

Antibody data

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Validation data

NEFH protein structure - LS-C204550 shown in red.

Enhanced validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Mixed neuron/glial cultures stained with NEFH / NF-H antibody (green) and also stained with rabbit polyclonal antibody to neurofilament NF-L RPCA-NF-L (red). The NF-L antibody stains neurofilaments in both axons and dendrites, and so can reveal neuronal cell bodies, while NEFH / NF-H antibody binds to only heavily phosphorylated forms of NF-H which are localized to mature axonal neurofilaments. In this image a few axons course from left to right and top to bottom- since they contain both NF-L and phosphorylated NF-H they appear golden in color. Blue shows the distribution of DNA.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Mixed neuron/glial cultures stained with NEFH / NF-H antibody (green) and also stained with rabbit polyclonal antibody to neurofilament NF-L RPCA-NF-L (red). The NF-L antibody stains neurofilaments in both axons and dendrites, and so can reveal neuronal cell bodies, while NEFH / NF-H antibody binds to only heavily phosphorylated forms of NF-H which are localized to mature axonal neurofilaments. In this image a few axons course from left to right and top to bottom- since they contain both NF-L and phosphorylated NF-H they appear golden in color. Blue shows the distribution of DNA.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: Mixed neuron/glial cultures stained with NEFH / NF-H antibody (green) and also stained with rabbit polyclonal antibody to neurofilament NF-L RPCA-NF-L (red). The NF-L antibody stains neurofilaments in both axons and dendrites, and so can reveal neuronal cell bodies, while NEFH / NF-H antibody binds to only heavily phosphorylated forms of NF-H which are localized to mature axonal neurofilaments. In this image a few axons course from left to right and top to bottom- since they contain both NF-L and phosphorylated NF-H they appear golden in color. Blue shows the distribution of DNA.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: Mixed neuron/glial cultures stained with NEFH / NF-H antibody (green) and also stained with rabbit polyclonal antibody to neurofilament NF-L RPCA-NF-L (red). The NF-L antibody stains neurofilaments in both axons and dendrites, and so can reveal neuronal cell bodies, while NEFH / NF-H antibody binds to only heavily phosphorylated forms of NF-H which are localized to mature axonal neurofilaments. In this image a few axons course from left to right and top to bottom- since they contain both NF-L and phosphorylated NF-H they appear golden in color. Blue shows the distribution of DNA.