Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- NBP2-47834-0.1mg - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal NF-H Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified. This MAb reacts with a 200kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). It reacts specifically with the phosphorylated KSP/KEP segment at the C-terminus of the heavy subunit (NF-H) of neurofilaments. After dephosphorylation of neurofilaments with alkaline phosphatase, this Ab no longer binds. Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.
- Reactivity
- Human, Mouse, Rat, Chicken/Avian, Feline, Guinea Pig, Porcine, Rabbit, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NF-H Antibody (NE14) - Azide and BSA Free [NBP2-47834] - Western Blot Analysis of human Brain tissue lysate using NF-H Antibody (NE14).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NF-H Antibody (NE14) - Azide and BSA Free [NBP2-47834] - Human Cerebellum stained with Neurofilament Monoclonal Antibody (NE14).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NF-H Antibody (NE14) - Azide and BSA Free [NBP2-47834] - Rat Cerebellum stained with Neurofilament Monoclonal Antibody (NE14).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: NF-H Antibody (NE14) - Azide and BSA Free [NBP2-47834] - Flow Cytometric Analysis of HEK293 cells using NF-H Antibody (NE14).followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).