835603

antibody from BioLegend

Targeting: NEFH NF-H, NFH

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

835603

Provider

BioLegend

Proper citation

BioLegend Cat#835603, RRID:AB_2715860

Product name

Purified anti-Neurofilament H & M (NF-H/NF-M), Hypophosphorylated

Antibody type

Monoclonal

Reactivity

Human, Mouse, Rat, Other

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

SMI 35

Vial size

25 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Immunohistochemistry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Immunostaining of formalin-fixed paraffin-embedded human cerebellum tissue with clone SMI 35. Antigen retrieval was performed using BioLegend’s Retrieve-All Antigen Unmasking System 3: Acidic, 10X and was followed by incubation with the primary antibody at 0.5µg/ml for 60 minutes at room temperature. BioLegend’s Ultra Streptavidin (USA) HRP Kit (Multi-Species) was used for detection and then followed by counterstaining with hematoxylin.

Conjugate

Unconjugated