Antibody data
- Antibody Data
- Antigen structure
- References [24]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- A05307 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Nefh Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for Nefh detection. Tested with WB, IHC-P, FCM, Direct ELISA in Mouse;Rat.
- Reactivity
- Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Transplantation of MiR-28-5p-Modified BMSCs Promotes Functional Recovery After Spinal Cord Injury.
Dexpramipexole Attenuates White Matter Injury to Facilitate Locomotion and Motor Coordination Recovery via Reducing Ferroptosis after Intracerebral Hemorrhage.
Nerve regeneration in rat peripheral nerve allografts: An assessment of the role of endogenous neurotrophic factors in nerve cryopreservation and regeneration.
3D printable Sodium alginate-Matrigel (SA-MA) hydrogel facilitated ectomesenchymal stem cells (EMSCs) neuron differentiation.
Biocompatible Chitin Hydrogel Incorporated with PEDOT Nanoparticles for Peripheral Nerve Repair.
Netrin-1 Promotes Synaptic Formation and Axonal Regeneration via JNK1/c-Jun Pathway after the Middle Cerebral Artery Occlusion.
The deletion of dicer in mature myelinating glial cells causes progressive axonal degeneration but not overt demyelination in adult mice.
MicroRNA-182 Alleviates Neuropathic Pain by Regulating Nav1.7 Following Spared Nerve Injury in Rats.
Gene silencing NMII promotes axonal regeneration against contusive spinal cord injury in rats.
Apolipoprotein E 4 triggers multiple pathway-mediated Ca2+ overload, causes CaMK II phosphorylation abnormity and aggravates oxidative stress caused cerebral cortical neuron damage.
Changes in the Expression of miR-34a and its Target Genes Following Spinal Cord Injury In Rats.
Increased Expression and Cellular Localization of P2X7R in Cortical Lesions of Patients With Focal Cortical Dysplasia.
Downregulation of CD47 and CD200 in patients with focal cortical dysplasia type IIb and tuberous sclerosis complex.
Altered expression of neurofilament 200 and amyloid-β peptide (1-40) in a rat model of chronic cerebral hypoperfusion.
Human mesenchymal stem cells improve the neurodegeneration of femoral nerve in a diabetic foot ulceration rats.
Endogenous subventricular zone neural progenitors contribute to the formation and hyperexcitability of experimental model of focal microgyria.
Improved retinal ganglion cell survival through retinal microglia suppression by a chinese herb extract, triptolide, in the DBA/2J mouse model of glaucoma.
The interleukin 17 system in cortical lesions in focal cortical dysplasias.
Effect of acupuncture on proliferation and differentiation of neural stem cells in brain tissues of rats with traumatic brain injury.
Amelioration of β-amyloid-induced cognitive dysfunction and hippocampal axon degeneration by curcumin is associated with suppression of CRMP-2 hyperphosphorylation.
Bone marrow mesenchymal stem cells and electroacupuncture downregulate the inhibitor molecules and promote the axonal regeneration in the transected spinal cord of rats.
Cograft of neural stem cells and schwann cells overexpressing TrkC and neurotrophin-3 respectively after rat spinal cord transection.
Implantation of adult bone marrow-derived mesenchymal stem cells transfected with the neurotrophin-3 gene and pretreated with retinoic acid in completely transected spinal cord.
Vaccination with dendritic cells pulsed with homogenate protein of spinal cord promotes functional recovery from spinal cord injury in mice.
Li Z, Su H, Lin G, Wang K, Huang Y, Wen Y, Luo D, Hou Y, Cao X, Weng J, Lin D, Wang L, Li X
Molecular neurobiology 2024 Apr;61(4):2197-2214
Molecular neurobiology 2024 Apr;61(4):2197-2214
Dexpramipexole Attenuates White Matter Injury to Facilitate Locomotion and Motor Coordination Recovery via Reducing Ferroptosis after Intracerebral Hemorrhage.
Wang B, Zhang X, Zhong J, Wang S, Zhang C, Li M, Hu Q, Wang S, Chen L, Chen W, Ge H, Feng H
Oxidative medicine and cellular longevity 2022;2022:6160701
Oxidative medicine and cellular longevity 2022;2022:6160701
Nerve regeneration in rat peripheral nerve allografts: An assessment of the role of endogenous neurotrophic factors in nerve cryopreservation and regeneration.
Zhang S, Zhou Y, Xian H, Shi Y, Liu Y, Li Z, Huang Y
The European journal of neuroscience 2022 Apr;55(8):1895-1916
The European journal of neuroscience 2022 Apr;55(8):1895-1916
3D printable Sodium alginate-Matrigel (SA-MA) hydrogel facilitated ectomesenchymal stem cells (EMSCs) neuron differentiation.
Li Y, Cao X, Deng W, Yu Q, Sun C, Ma P, Shao F, Yusif MM, Ge Z, Wang K, Li R, Yu J, Xu X
Journal of biomaterials applications 2021 Jan;35(6):709-719
Journal of biomaterials applications 2021 Jan;35(6):709-719
Biocompatible Chitin Hydrogel Incorporated with PEDOT Nanoparticles for Peripheral Nerve Repair.
Huang L, Yang X, Deng L, Ying D, Lu A, Zhang L, Yu A, Duan B
ACS applied materials & interfaces 2021 Apr 14;13(14):16106-16117
ACS applied materials & interfaces 2021 Apr 14;13(14):16106-16117
Netrin-1 Promotes Synaptic Formation and Axonal Regeneration via JNK1/c-Jun Pathway after the Middle Cerebral Artery Occlusion.
Zheng M, Chen R, Chen H, Zhang Y, Chen J, Lin P, Lan Q, Yuan Q, Lai Y, Jiang X, Pan X, Liu N
Frontiers in cellular neuroscience 2018;12:13
Frontiers in cellular neuroscience 2018;12:13
The deletion of dicer in mature myelinating glial cells causes progressive axonal degeneration but not overt demyelination in adult mice.
Li T, Wang J, Wang H, Yang Y, Wang S, Huang N, Wang F, Gao X, Niu J, Li Z, Mei F, Xiao L
Glia 2018 Sep;66(9):1960-1971
Glia 2018 Sep;66(9):1960-1971
MicroRNA-182 Alleviates Neuropathic Pain by Regulating Nav1.7 Following Spared Nerve Injury in Rats.
Cai W, Zhao Q, Shao J, Zhang J, Li L, Ren X, Su S, Bai Q, Li M, Chen X, Wang J, Cao J, Zang W
Scientific reports 2018 Nov 13;8(1):16750
Scientific reports 2018 Nov 13;8(1):16750
Gene silencing NMII promotes axonal regeneration against contusive spinal cord injury in rats.
Ning G, Liu Y, Xu H, Li Y, Wu H, Wang X, Feng S
International journal of clinical and experimental pathology 2017;10(11):11345-11352
International journal of clinical and experimental pathology 2017;10(11):11345-11352
Apolipoprotein E 4 triggers multiple pathway-mediated Ca2+ overload, causes CaMK II phosphorylation abnormity and aggravates oxidative stress caused cerebral cortical neuron damage.
Xu D, Peng Y
European review for medical and pharmacological sciences 2017 Dec;21(24):5717-5728
European review for medical and pharmacological sciences 2017 Dec;21(24):5717-5728
Changes in the Expression of miR-34a and its Target Genes Following Spinal Cord Injury In Rats.
Chen Y, Cao S, Xu P, Han W, Shan T, Pan J, Lin W, Chen X, Wang X
Medical science monitor : international medical journal of experimental and clinical research 2016 Oct 25;22:3981-3993
Medical science monitor : international medical journal of experimental and clinical research 2016 Oct 25;22:3981-3993
Increased Expression and Cellular Localization of P2X7R in Cortical Lesions of Patients With Focal Cortical Dysplasia.
Wei YJ, Guo W, Sun FJ, Fu WL, Zheng DH, Chen X, Li S, Zang ZL, Zhang CQ, Liu SY, Yang H
Journal of neuropathology and experimental neurology 2016 Jan;75(1):61-8
Journal of neuropathology and experimental neurology 2016 Jan;75(1):61-8
Downregulation of CD47 and CD200 in patients with focal cortical dysplasia type IIb and tuberous sclerosis complex.
Sun FJ, Zhang CQ, Chen X, Wei YJ, Li S, Liu SY, Zang ZL, He JJ, Guo W, Yang H
Journal of neuroinflammation 2016 Apr 19;13(1):85
Journal of neuroinflammation 2016 Apr 19;13(1):85
Altered expression of neurofilament 200 and amyloid-β peptide (1-40) in a rat model of chronic cerebral hypoperfusion.
Liang W, Zhang W, Zhao S, Li Q, Liang H, Ceng R
Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology 2015 May;36(5):707-12
Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology 2015 May;36(5):707-12
Human mesenchymal stem cells improve the neurodegeneration of femoral nerve in a diabetic foot ulceration rats.
Xia N, Xu JM, Zhao N, Zhao QS, Li M, Cheng ZF
Neuroscience letters 2015 Jun 15;597:84-9
Neuroscience letters 2015 Jun 15;597:84-9
Endogenous subventricular zone neural progenitors contribute to the formation and hyperexcitability of experimental model of focal microgyria.
Shu HF, Kuang YQ, Liu SY, Yu SX, Zhang CQ, Zheng DH, Gu JW, Yang H
Journal of molecular neuroscience : MN 2014 Apr;52(4):586-97
Journal of molecular neuroscience : MN 2014 Apr;52(4):586-97
Improved retinal ganglion cell survival through retinal microglia suppression by a chinese herb extract, triptolide, in the DBA/2J mouse model of glaucoma.
Yang F, Wu L, Guo X, Wang D, Li Y
Ocular immunology and inflammation 2013 Oct;21(5):378-89
Ocular immunology and inflammation 2013 Oct;21(5):378-89
The interleukin 17 system in cortical lesions in focal cortical dysplasias.
He JJ, Li S, Shu HF, Yu SX, Liu SY, Yin Q, Yang H
Journal of neuropathology and experimental neurology 2013 Feb;72(2):152-63
Journal of neuropathology and experimental neurology 2013 Feb;72(2):152-63
Effect of acupuncture on proliferation and differentiation of neural stem cells in brain tissues of rats with traumatic brain injury.
Zhang YM, Zhang YQ, Cheng SB, Chen SX, Chen AL, Tang CZ
Chinese journal of integrative medicine 2013 Feb;19(2):132-6
Chinese journal of integrative medicine 2013 Feb;19(2):132-6
Amelioration of β-amyloid-induced cognitive dysfunction and hippocampal axon degeneration by curcumin is associated with suppression of CRMP-2 hyperphosphorylation.
Wang Y, Yin H, Li J, Zhang Y, Han B, Zeng Z, Qiao N, Cui X, Lou J, Li J
Neuroscience letters 2013 Dec 17;557 Pt B:112-7
Neuroscience letters 2013 Dec 17;557 Pt B:112-7
Bone marrow mesenchymal stem cells and electroacupuncture downregulate the inhibitor molecules and promote the axonal regeneration in the transected spinal cord of rats.
Ding Y, Yan Q, Ruan JW, Zhang YQ, Li WJ, Zeng X, Huang SF, Zhang YJ, Wang S, Dong H, Zeng YS
Cell transplantation 2011;20(4):475-91
Cell transplantation 2011;20(4):475-91
Cograft of neural stem cells and schwann cells overexpressing TrkC and neurotrophin-3 respectively after rat spinal cord transection.
Wang JM, Zeng YS, Wu JL, Li Y, Teng YD
Biomaterials 2011 Oct;32(30):7454-68
Biomaterials 2011 Oct;32(30):7454-68
Implantation of adult bone marrow-derived mesenchymal stem cells transfected with the neurotrophin-3 gene and pretreated with retinoic acid in completely transected spinal cord.
Zhang W, Yan Q, Zeng YS, Zhang XB, Xiong Y, Wang JM, Chen SJ, Li Y, Bruce IC, Wu W
Brain research 2010 Nov 4;1359:256-71
Brain research 2010 Nov 4;1359:256-71
Vaccination with dendritic cells pulsed with homogenate protein of spinal cord promotes functional recovery from spinal cord injury in mice.
Liu M, Zhao J, Liang H, Bian X
Spinal cord 2009 May;47(5):360-6
Spinal cord 2009 May;47(5):360-6
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of Neuro-2a cells using anti-Nefh antibody (A05307). Overlay histogram showing Neuro-2a cells stained with A05307 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Nefh Antibody (A05307, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
- Additional image