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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
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- Product number
- MA1-10441 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NF-H Monoclonal Antibody (NF-05)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- This antibody recognizes a nonphosphorylated epitope of neurofilament heavy protein (NF-H), a 210 kDa intracellular structural protein of Intermediate Filament Proteins family. It will not cross-react with mouse.
- Reactivity
- Human, Rat, Porcine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- NF-05
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- 4° C, do not freeze
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blotting analysis of neurofilament heavy protein in porcine brain lysate (reducing conditions) by mouse monoclonal NF-05.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blotting analysis of human neurofilament H protein using mouse monoclonal antibody NF-05 on lysates of HEK-293 cell line, human brain lysate, and MCF-7 cell line (neurofilament non-expressing cell line; negative control) under reducing conditions. Nitrocellulose membrane was probed with 2µg/mL of mouse anti-neurofilament H monoclonal antibody (Product # MA1-10441) followed by IRDye800-conjugated anti-mouse secondary antibody. Neurofilament H was detected around 180 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blotting analysis of neurofilament heavy protein in porcine brain lysate (reducing conditions) by mouse NF-05 Monoclonal antibody (Product # MA1-10441).
