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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- MA1-19172 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NF-H Monoclonal Antibody (NF-01)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- This antibody recognizes a phosphorylated epitope on heavy neurofilament protein (210 kDa) of various species.
- Reactivity
- Human, Mouse, Rat, Porcine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- NF-01
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- 4° C, do not freeze
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blotting analysis of human neurofilament H protein using mouse Monoclonal antibody NF-01 (Product # MA1-19172) on lysates of HEK-293 cell line, human brain lysate, and MCF-7 cell line (neurofilament non-expressing cell line; negative control) under reducing conditions. Nitrocellulose membrane was probed with 2µg/mL of mouse anti-neurofilament H monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. Neurofilament H was detected around 180 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry staining of human cerebellum (paraffin-embedded sections) with anti-neurofilament heavy protein (NF-01) Monoclonal antibody (Product # MA1-19172).
