PA1-10002

antibody from Invitrogen Antibodies

Targeting: NEFH NF-H, NFH

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-10002

Provider

Invitrogen Antibodies

Product name

NF-H Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Purifed from natural sources

Description

This antibody reacts primarily with the phosphorylated axonal form of NF-H and shows some cross-reactivity with phosphorylated NF-M, which has similar phosphorylation sites to NF-H.

Reactivity

Human, Mouse, Rat, Bovine, Canine, Porcine

Host

Chicken/Avian

Isotype

IgY

Vial size

50 µL

Concentration

Conc. Not Determined

Storage

4° C

References

Submitted references

Myelination, axonal loss and Schwann cell characteristics in axonal polyneuropathy compared to controls.

Placheta-Györi E, Brandstetter LM, Zemann-Schälss J, Wolf S, Radtke C
PloS one 2021;16(11):e0259654

---

Nano-in-Micro-Particles Consisting of PLGA Nanoparticles Embedded in Chitosan Microparticles via Spray-Drying Enhances Their Uptake in the Olfactory Mucosa.

Spindler LM, Feuerhake A, Ladel S, Günday C, Flamm J, Günday-Türeli N, Türeli E, Tovar GEM, Schindowski K, Gruber-Traub C
Frontiers in pharmacology 2021;12:732954

---

Epitope-preserving magnified analysis of proteome (eMAP).

Park J, Khan S, Yun DH, Ku T, Villa KL, Lee JE, Zhang Q, Park J, Feng G, Nedivi E, Chung K
Science advances 2021 Nov 12;7(46):eabf6589

---

Endoplasmic reticulum stress in the dorsal root ganglia regulates large-conductance potassium channels and contributes to pain in a model of multiple sclerosis.

Yousuf MS, Samtleben S, Lamothe SM, Friedman TN, Catuneanu A, Thorburn K, Desai M, Tenorio G, Schenk GJ, Ballanyi K, Kurata HT, Simmen T, Kerr BJ
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 Sep;34(9):12577-12598

---

Sensory Neurons of the Dorsal Root Ganglia Become Hyperexcitable in a T-Cell-Mediated MOG-EAE Model of Multiple Sclerosis.

Yousuf MS, Noh MC, Friedman TN, Zubkow K, Johnson JC, Tenorio G, Kurata HT, Smith PA, Kerr BJ
eNeuro 2019 Mar-Apr;6(2)

---

Murine Retinal Citrullination Declines With Age and is Mainly Dependent on Peptidyl Arginine Deiminase 4 (PAD4).

Hollingsworth TJ, Radic MZ, Beranova-Giorgianni S, Giorgianni F, Wang Y, Iannaccone A
Investigative ophthalmology & visual science 2018 Aug 1;59(10):3808-3815

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NF-H in spinal cord lysates using a polyclonal antibody (Product # PA1-10002) at a dilution of 1:20,000 as seen in green. 1) protein standard (red), 2) rat, 3) mouse, and 4) cow spinal cord. Strong band at about 200-220kDa corresponds to the phosphorylated from of NF-H. The protein from different species is known to have different SDS-PAGE molecular weights, with large species generally expressing larger proteins. Smaller proteolytic fragments of NF-H are also detected in spinal cord preparations with this antibody. The antibody does not recognize non-phosphorylated forms of NF-H (not shown).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of NEFH using a polyclonal antibody (Product # PA1-10002).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of NEFH using a polyclonal antibody (Product # PA1-10002).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

FIGURE 5 Permeation of PLGA nanoparticles (520 nm; red) (A-E) and PLGA-chitosan nano-in-micro particles (NiMPs) (F-J) . Maximum intensity projection (MIP) of Confocal Laser Scanning Microscopy (CLSM) images stitched together from a z-stack, taken 15 min after application to the apical side of porcine olfactory mucosa. Cell nuclei stained with DAPI (blue). Red arrows mark nanoparticles (red) within the tissue. CD14 (green) labelled with anti-mouse Alexa Fluor (r) 647 secondary antibody (A, B, F, G) . NF-H (cyan) labelled with anti-chicken FITC secondary antibody (D, E) . Chitosan labeled with fluorescein (cyan) (H) . Overlapping signals of red and green appear yellow (F) due to co-localization of PLGA nanoparticles (red) and chitosan (green). PLGA nanoparticles (single red channel) displayed in Supplementary Figure S6B, C . LP, Lamina propria; BL, Basal cell layer; PL, Particle layer; EL, Epithelial cell layer.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

10.1371/journal.pone.0259654.g002 Fig 2 Immunohistochemistry of nerve sections. Area fraction of immunosignals in nerve biopsies obtained from PNP patients compared to controls. S100 positive Schwann cells (A) were significantly reduced in PNP. Neurofilament-H (B) was highly significantly lower in the PNP group. There was reduced immunoreactivity to the myelination parameter myelin basic protein in PNP patients (C), while immature p75-positive Schwann cells were increased (D). PNP : Polyneuropathy .

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig 3 Immunofluorescent staining. S100 positive Schwann cells (red), neurofilament-H (NF-H; purple) and myelin basic protein (MBP; green) are reduced in polyneuropathy (PNP). In the merged panel, cell bodies are counter-stained with DAPI (blue). Imaged at 20x magnification under equal exposure times; scale bar: 100 mum.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 2. eMAP offers high expansion factors by recursive embedding while preserving the spatial organization of molecules. ( A ) Schematic of the recursive embedding of an eMAP-processed tissue. ( B ) Individual synapses in eMAP-processed, 10x linearly expanded mouse brain tissues visualized by different sets of Bassoon and PSD-95 antibodies: Bassoon SYSY 141004 and PSD-95 NeuroMab 75-028 (top), and Bassoon SYSY 141003 and PSD-95 NeuroMab 75-028 (bottom). Scale bars, 200 nm (2 mum in expanded). ( C ) 3D-rendered image of a 50-mum-thick (5 mum before expansion) mouse brain slice stained with anti-YFP (white), Bassoon (magenta), and PSD-95 (green) antibodies. Scale bars, 500 nm (5 mum in expanded). (A) and (B) are inset images of respective dendritic spines highlighted in the 3D-rendered image. Scale bars, 200 nm (2 mum in expanded). ( D ) Images showing Lectin-stained (top) and NF-H-stained (bottom) tissues before and after expansion. Scale bars, 500 mum (Lectin) and 50 mum (NF-H). The plots on the right show the root mean square error (RMSE) at different measurement lengths mesoscale (top) and microscale (bottom). ( E ) Individual synapses in eMAP-processed, 10x linearly expanded marmoset brain tissues visualized by different sets of antibodies. Scale bars, 200 nm (2 mum in expanded). ( F ) Antibody compatibility in eMAP-processed marmoset brain tissue for the 27 SyPs in Fig. 1D .