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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [13]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NB300-135 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-135, RRID:AB_10001839
- Product name
- Rabbit Polyclonal NF-H Antibody
- Antibody type
- Polyclonal
- Description
- Unpurified. Phosphorylated and non-phosphorylated forms of 200kDa Neurofilament Heavy
- Reactivity
- Human, Mouse, Rat, Bovine, Porcine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.05 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Innervation of the hip joint capsular complex: A systematic review of histological and immunohistochemical studies and their clinical implications for contemporary treatment strategies in total hip arthroplasty.
An effective erythropoietin dose regimen protects against severe nerve injury-induced pathophysiological changes with improved neural gene expression and enhances functional recovery.
Long-term culturing of porcine nodose ganglia.
Differential Hyperphosphorylation of Tau-S199, -T231 and -S396 in Organotypic Brain Slices of Alzheimer Mice. A Model to Study Early Tau Hyperphosphorylation Using Okadaic Acid.
Primary mouse brain pericytes isolated from transgenic Alzheimer mice spontaneously differentiate into a CD11b+ microglial-like cell type in vitro.
Generation of induced pluripotent stem cell line from foreskin fibroblasts.
Maintenance of Taste Organs Is Strictly Dependent on Epithelial Hedgehog/GLI Signaling.
Hedgehog pathway blockade with the cancer drug LDE225 disrupts taste organs and taste sensation.
Oligodendrogenesis and myelinogenesis during postnatal development effect of glatiramer acetate.
Multiple Shh signaling centers participate in fungiform papilla and taste bud formation and maintenance.
Neurofilament protein levels: quantitative analysis in essential tremor cerebellar cortex.
Apelin, an endogenous neuronal peptide, protects hippocampal neurons against excitotoxic injury.
Human immunodeficiency virus (HIV)-induced neurotoxicity: roles for the NMDA receptor subtypes.
Tomlinson J, Zwirner J, Ondruschka B, Prietzel T, Hammer N
PloS one 2020;15(2):e0229128
PloS one 2020;15(2):e0229128
An effective erythropoietin dose regimen protects against severe nerve injury-induced pathophysiological changes with improved neural gene expression and enhances functional recovery.
Govindappa PK, Talukder MAH, Gurjar AA, Hegarty JP, Elfar JC
International immunopharmacology 2020 Mar 3;82:106330
International immunopharmacology 2020 Mar 3;82:106330
Long-term culturing of porcine nodose ganglia.
Kuan SP, Atanasova KR, Guevara MV, Collins EN, Reznikov LR
Journal of neuroscience methods 2020 Feb 15;332:108546
Journal of neuroscience methods 2020 Feb 15;332:108546
Differential Hyperphosphorylation of Tau-S199, -T231 and -S396 in Organotypic Brain Slices of Alzheimer Mice. A Model to Study Early Tau Hyperphosphorylation Using Okadaic Acid.
Foidl BM, Humpel C
Frontiers in aging neuroscience 2018;10:113
Frontiers in aging neuroscience 2018;10:113
Primary mouse brain pericytes isolated from transgenic Alzheimer mice spontaneously differentiate into a CD11b+ microglial-like cell type in vitro.
Hutter-Schmid B, Humpel C
Experimental gerontology 2018 Oct 2;112:30-37
Experimental gerontology 2018 Oct 2;112:30-37
Generation of induced pluripotent stem cell line from foreskin fibroblasts.
Skrzypczyk A, Giri S, Bader A
Stem cell research 2016 Nov;17(3):572-575
Stem cell research 2016 Nov;17(3):572-575
Maintenance of Taste Organs Is Strictly Dependent on Epithelial Hedgehog/GLI Signaling.
Ermilov AN, Kumari A, Li L, Joiner AM, Grachtchouk MA, Allen BL, Dlugosz AA, Mistretta CM
PLoS genetics 2016 Nov;12(11):e1006442
PLoS genetics 2016 Nov;12(11):e1006442
Hedgehog pathway blockade with the cancer drug LDE225 disrupts taste organs and taste sensation.
Kumari A, Ermilov AN, Allen BL, Bradley RM, Dlugosz AA, Mistretta CM
Journal of neurophysiology 2015 Feb 1;113(3):1034-40
Journal of neurophysiology 2015 Feb 1;113(3):1034-40
Oligodendrogenesis and myelinogenesis during postnatal development effect of glatiramer acetate.
From R, Eilam R, Bar-Lev DD, Levin-Zaidman S, Tsoory M, LoPresti P, Sela M, Arnon R, Aharoni R
Glia 2014 Apr;62(4):649-65
Glia 2014 Apr;62(4):649-65
Multiple Shh signaling centers participate in fungiform papilla and taste bud formation and maintenance.
Liu HX, Ermilov A, Grachtchouk M, Li L, Gumucio DL, Dlugosz AA, Mistretta CM
Developmental biology 2013 Oct 1;382(1):82-97
Developmental biology 2013 Oct 1;382(1):82-97
Neurofilament protein levels: quantitative analysis in essential tremor cerebellar cortex.
Louis ED, Ma K, Babij R, Cortés E, Liem RK, Vonsattel JP, Faust PL
Neuroscience letters 2012 Jun 14;518(1):49-54
Neuroscience letters 2012 Jun 14;518(1):49-54
Apelin, an endogenous neuronal peptide, protects hippocampal neurons against excitotoxic injury.
O'Donnell LA, Agrawal A, Sabnekar P, Dichter MA, Lynch DR, Kolson DL
Journal of neurochemistry 2007 Sep;102(6):1905-1917
Journal of neurochemistry 2007 Sep;102(6):1905-1917
Human immunodeficiency virus (HIV)-induced neurotoxicity: roles for the NMDA receptor subtypes.
O'Donnell LA, Agrawal A, Jordan-Sciutto KL, Dichter MA, Lynch DR, Kolson DL
The Journal of neuroscience : the official journal of the Society for Neuroscience 2006 Jan 18;26(3):981-90
The Journal of neuroscience : the official journal of the Society for Neuroscience 2006 Jan 18;26(3):981-90
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NF-H Antibody [NB300-135] - Analysis of 200kDa Neurofilament Heavy expression in rat spinal cord extract. The first lane is Coomassie Brilliant Blue stained and the second lane is probed with rabbit anti-Neurofilament Heavy antibody NB300-135. The NF-H corresponds to a weight of 200kDa.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NF-H Antibody [NB300-135] - Different tissue lysates using rabbit pAb to NF-H, dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord [4] mouse brain, and [5] mouse spinal cord lysate. Strong band at about 220 kDa corresponds to the phosphorylated axonal form of the NF-H subunit. Smaller proteolytic fragments of NF-H are also detected with RPCA-NF-H antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: NF-H Antibody [NB300-135] - Mouse hippocampus section stained with rabbit pAb to NF-H, dilution 1:2,000 in red, and costained with mouse mAb to myelin basic protein (MBP), dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The NF-H antibody labels a network of axons of different neurons, while the MBP antibody stains myelin sheath around these axons.
