MA1071

antibody from Boster Biological Technology

Targeting: NEFH NF-H, NFH

Western blot (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1071

Provider

Boster Biological Technology

Product name

Anti-NF200 Antibody (monoclonal)

Antibody type

Monoclonal

Antigen

C-terminal segment of enzymatically dephosphorylated pig Neurofilament 200.

Description

Ascites

Reactivity

Human, Mouse, Rat

Vial size

100μg/vial

Concentration

Lyophilized

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100μg/ml.

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

IHC analysis of NEFH using anti- NEFH antibody (MA1071). NEFH was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti- NEFH Antibody (MA1071) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

Additional image