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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- GTX101807 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX101807, RRID:AB_1240813
- Product name
- Fibrillarin antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references A high-throughput pipeline for validation of antibodies.
Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F
Nature methods 2018 Nov;15(11):909-912
Nature methods 2018 Nov;15(11):909-912
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Fibrillarin antibody detects FBL protein by Western blot analysis.A. 30 ?g Rat2 whole cell lysate/extract12 % SDS-PAGEFibrillarin antibody (GTX101807) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: 293T B: H1299 12% SDS PAGE GTX101807 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A:NIH-3T3 10% SDS PAGE GTX101807 diluted at 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Fibrillarin(GTX101807) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Fibrillarin antibody immunoprecipitates FBL protein in IP experiments.IP samples: HeLa nuclear extractA. 35 £gg HeLa nuclear extractB. Control with 4 £gg of preimmune Rabbit IgGC. Immunoprecipitation of FBL protein by 4 £gg Fibrillarin antibody (GTX101807)10 % SDS-PAGEThe immunoprecipitated FBL protein was detected by Fibrillarin antibody (GTX101807) diluted at 1:1000.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
