NB300-269

antibody from Novus Biologicals

Targeting: FBL FIB, FLRN, Nop1, RNU3IP1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB300-269

Provider

Novus Biologicals

Proper citation

Novus Cat#NB300-269, RRID:AB_2100980

Product name

Mouse Monoclonal Fibrillarin Antibody

Antibody type

Monoclonal

Description

Affinity purified.

Reactivity

Human, Mouse, Rat, Bovine, Chicken/Avian, Drosophila, Porcine, Yeast

Host

Mouse

Isotype

IgG

Vial size

0.25 ml

Concentration

1 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Integrative analyses of the RNA modification machinery reveal tissue- and cancer-specific signatures.

Begik O, Lucas MC, Liu H, Ramirez JM, Mattick JS, Novoa EM
Genome biology 2020 May 7;21(1):97

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Defects in CISD-1, a mitochondrial iron-sulfur protein, lower glucose level and ATP production in Caenorhabditis elegans.

Hsiung KC, Liu KY, Tsai TF, Yoshina S, Mitani S, Chin-Ming Tan B, Lo SJ
Biomedical journal 2020 Feb;43(1):32-43

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The Fml1-MHF complex suppresses inter-fork strand annealing in fission yeast.

Wong IN, Neo JP, Oehler J, Schafhauser S, Osman F, Carr SB, Whitby MC
eLife 2019 Dec 19;8

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Nucleolar fibrillarin is an evolutionarily conserved regulator of bacterial pathogen resistance.

Tiku V, Kew C, Mehrotra P, Ganesan R, Robinson N, Antebi A
Nature communications 2018 Sep 6;9(1):3607

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Nucleolar expansion and elevated protein translation in premature aging.

Buchwalter A, Hetzer MW
Nature communications 2017 Aug 30;8(1):328

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Small nucleoli are a cellular hallmark of longevity.

Tiku V, Jain C, Raz Y, Nakamura S, Heestand B, Liu W, Späth M, Suchiman HED, Müller RU, Slagboom PE, Partridge L, Antebi A
Nature communications 2017 Aug 30;8:16083

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The arrangement of Brachypodium distachyon chromosomes in interphase nuclei.

Robaszkiewicz E, Idziak-Helmcke D, Tkacz MA, Chrominski K, Hasterok R
Journal of experimental botany 2016 Oct;67(18):5571-5583

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Characterization of Aes nuclear foci in colorectal cancer cells.

Itatani Y, Sonoshita M, Kakizaki F, Okawa K, Stifani S, Itoh H, Sakai Y, Taketo MM
Journal of biochemistry 2016 Jan;159(1):133-40

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Nucleophosmin integrates within the nucleolus via multi-modal interactions with proteins displaying R-rich linear motifs and rRNA.

Mitrea DM, Cika JA, Guy CS, Ban D, Banerjee PR, Stanley CB, Nourse A, Deniz AA, Kriwacki RW
eLife 2016 Feb 2;5

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The TALE transcription factor homothorax functions to assemble heterochromatin during Drosophila embryogenesis.

Zaballos MA, Cantero W, Azpiazu N
PloS one 2015;10(3):e0120662

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Elevated snoRNA biogenesis is essential in breast cancer.

Su H, Xu T, Ganapathy S, Shadfan M, Long M, Huang TH, Thompson I, Yuan ZM
Oncogene 2014 Mar 13;33(11):1348-58

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Mutation of a Nopp140 gene dao-5 alters rDNA transcription and increases germ cell apoptosis in C. elegans.

Lee CC, Tsai YT, Kao CW, Lee LW, Lai HJ, Ma TH, Chang YS, Yeh NH, Lo SJ
Cell death & disease 2014 Apr 10;5(4):e1158

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Large-scale organization of ribosomal DNA chromatin is regulated by Tip5.

Zillner K, Filarsky M, Rachow K, Weinberger M, Längst G, Németh A
Nucleic acids research 2013 May 1;41(10):5251-62

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Pinpointing the expression of piRNAs and function of the PIWI protein subfamily during spermatogenesis in the mouse.

Beyret E, Lin H
Developmental biology 2011 Jul 15;355(2):215-26

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Fibrillarin Antibody (38F3) [NB300-269] - Western blot analysis of Fibrillarin expression on yeast protein extracts using NB300-269.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Fibrillarin Antibody (38F3) [NB300-269] - of whole yeast protein extracts with a collection of our antibodies. The blot for Fibrillarin Antibody (38F3) is in the indicated lane, and the number indicates the SDS-PAGE molecular weight in kiloDaltons.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Fibrillarin Antibody (38F3) [NB300-269] - Analysis of lysates of cell fractions probed with Fibrillarin antibody, dilution 1:500 (Green): [1] protein standard, [2] C6 cytosol, [3] C6 nuclear, [4] HEK293 cytosol, [5] HEK293 nuclear, [6] NIH-3T3 cytosol and [7] NIH-3T3 nuclear fractions. The band at 37kDa corresponds to the fibrillarin protein detected exclusively in the nuclear fractions.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Fibrillarin Antibody (38F3) [NB300-269] - IHC staining of Fibrillarin in human ovarian cancer using DAB with hematoxylin counterstain.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: Fibrillarin Antibody (38F3) [NB300-269] - Fibrillarin antibody was tested at 1:400 in HEK293 cells using an Alexa Fluor 488 secondary (shown in green) alongside unstained cells (shown in red).