Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
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Validation data
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- Product number
- LS-B13537 - Provider product page
- Provider
- LSBio
- Product name
- IHC-plus™ FBL / FIB / Fibrillarin Antibody (aa1-50) LS-B13537
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store at 2-8°C for up to one year.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Detection of Human and Mouse Fibrillarin by Western Blot. Samples: Whole cell lysate from HeLa (15 and 50 ug), 293T (50 ug), Jurkat (50 ug), and mouse NIH3T3 (50 ug) cells. Antibodies: Affinity purified rabbit anti-Fibrillarin antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Detection of mouse PHAX by western blot. Samples: Whole cell lysate from mouse NIH 3T3 (15 and 50 µg) cells. Antibodies: Affinity purified rabbit anti-PHAX antibody used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Detection of human PHAX by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 µg), HeLa (50µg), and Jurkat (50µg) cells. Antibodies: Affinity purified rabbit anti-PHAX antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.