GTX15666

antibody from GeneTex

Targeting: ATM ATA, ATC, ATD, ATDC, TEL1, TELO1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

GTX15666

Provider

GeneTex

Product name

ATM (phospho Ser1981) antibody [10H11]

Antibody type

Monoclonal

Reactivity

Human, Mouse

Host

Mouse

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western blot analysis of Ser1981-phosphorylated ATM in gamma-irradiated HeLa cells.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescent analysis of Ser1981-phosphorylated ATM in control (left panel) or 100 mJ UV-stimulated 293 cells (right panel). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ATM (phospho Ser1981) antibody [10H11] at a dilution of 1:50 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a proper secondary antibody. Nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.