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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AP09424PU-S - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP09424PU-S, RRID:AB_2035152
- Product name
- anti ATM pSer1981
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide corresponding to a region near serine 1981 of human ATM conjugated to KLH using maleimide
- Reactivity
- Human
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 25 µl
- Concentration
- 1.0 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
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- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Lane 2 shows anti-ATM pS1981 polyclonal antibody detects ATM in Hydrogen Peroxide Stimulated Human Derived MCF-7 Whole Cell Lysate. Lane 1 shows a lysate from untreated MCF-7 cell lysate. The band corresponding to ATM (370 kDa) is indicated by an arrow. After blocking, the membrane was probed with a 1:1000 dilution of the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:5,000 dilution of IRDye(TM)800 conjugated Donkey anti-Rabbit IgG for 1h at room temperature. LICOR's Odyssey(R) Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
