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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- AP09424PU-S - Provider product page
- Provider
- OriGene
- Product name
- Sheep Polyclonal Anti-ATM Antibody, Aff - Purified
- Antibody type
- Polyclonal
- Description
- Sheep Polyclonal Anti-ATM Antibody, Aff - Purified
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 25ul
- Concentration
- 1.0 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Lane 2 shows anti-ATM pS1981 polyclonal antibody detects ATM in Hydrogen Peroxide Stimulated Human Derived MCF-7 Whole Cell Lysate. Lane 1 shows a lysate from untreated MCF-7 cell lysate. The band corresponding to ATM (370 kDa) is indicated by an arrow. After blocking, the membrane was probed with a 1:1000 dilution of the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:5,000 dilution of IRDye(TM)800 conjugated Donkey anti-Rabbit IgG for 1h at room temperature. LICOR's Odyssey(R) Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Schematic of ATM induction by DNA damage.
- Validation comment
- Assay
