Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- 14-9046-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-ATM (Ser1981) Monoclonal Antibody (10H11.E12), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This 10H11.E12 monoclonal antibody recognizes the human, mouse, and rat ataxia-telangiectasia (ATM) protein when phosphorylated on serine 1981. ATM belongs to the family of PI3 kinases and functions by coordinating cell cycle arrest and initiation of DNA repair through the phosphorylation of a multitude of substrates in response to DNA damage and oxidative stress. ATM exists as dimers or multimers in its inactive state but in response to DNA breaks, becomes activated through formation of monomers and autophosphorylation. Activated ATM is recruited to the site of DNA double strand breaks where it halts the cell cycle and initiates DNA repair through the phosphorylation of downstream DNA damage response pathway members. Loss of functional activity of ATM results in ataxia-telangiectasia (AT), a disease characterized by early onset neurodegeneration and predisposition to cancer. AT patients are immunodeficient, radiosensitive, and have an increased risk of certain cancer types such as lymphoma and leukemia. Applications Reported: This 10H11.E12 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, immunoprecipitation, western blotting, immunocytochemistry, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. (Fluorochrome conjugated 10H11.E12 is recommended for use in intracellular flow cytometry.). Applications Tested: This 10H11.E12 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using either high or low pH antigen retrieval and can be used at less than or equal to 10 µg/mL. This 10H11.E12 antibody has been tested by immunocytochemistry of methanol or formaldehyde-fixed and permeabilized cells and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 10H11.E12
- Vial size
- 25 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references Stromal fibroblasts support dendritic cells to maintain IL-23/Th17 responses after exposure to ionizing radiation.
Mechanisms of ATM Activation.
Autophosphorylation and ATM activation: additional sites add to the complexity.
Disappearance of the telomere dysfunction-induced stress response in fully senescent cells.
Malecka A, Wang Q, Shah S, Sutavani RV, Spendlove I, Ramage JM, Greensmith J, Franks HA, Gough MJ, Saalbach A, Patel PM, Jackson AM
Journal of leukocyte biology 2016 Aug;100(2):381-9
Journal of leukocyte biology 2016 Aug;100(2):381-9
Mechanisms of ATM Activation.
Paull TT
Annual review of biochemistry 2015;84:711-38
Annual review of biochemistry 2015;84:711-38
Autophosphorylation and ATM activation: additional sites add to the complexity.
Kozlov SV, Graham ME, Jakob B, Tobias F, Kijas AW, Tanuji M, Chen P, Robinson PJ, Taucher-Scholz G, Suzuki K, So S, Chen D, Lavin MF
The Journal of biological chemistry 2011 Mar 18;286(11):9107-19
The Journal of biological chemistry 2011 Mar 18;286(11):9107-19
Disappearance of the telomere dysfunction-induced stress response in fully senescent cells.
Bakkenist CJ, Drissi R, Wu J, Kastan MB, Dome JS
Cancer research 2004 Jun 1;64(11):3748-52
Cancer research 2004 Jun 1;64(11):3748-52
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry of camptothecin-treated HeLa cells using 10 µg/mL of Anti-phospho-ATM (S1981) Purified, followed by F (ab')2 Anti-Mouse IgG eFluor® 570.Nuceli are stained with DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of formalin-fixed paraffin embedded human infiltrating ductile carcinoma using 10 µg/mL of Anti-phospho-ATM (S1981) Purified, followed by Anti-Mouse IgG Biotin, streptavidin HRP, and DAB visualization. Nuclei are counterstained with hematoxylin.