MA5-14871

antibody from Invitrogen Antibodies

Targeting: ATM ATA, ATC, ATD, ATDC, TEL1, TELO1

Western blot (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-14871

Provider

Invitrogen Antibodies

Product name

ATM Monoclonal Antibody (C.74.4)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

It is not recommended to aliquot this antibody.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Antibody clone number

C.74.4

Vial size

100 μL

Concentration

143 μg/mL

Storage

-20°C

References

Submitted references

Phosphorylation of BRCA1 by ATM upon double-strand breaks impacts ATM function in end-resection: A potential feedback loop.

Qi L, Chakravarthy R, Li MM, Deng CX, Li R, Hu Y
iScience 2022 Sep 16;25(9):104944

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Abrogation of ATM phosphorylation on BRCA1-S1152 reduces SKP2 recruitment and ATM-NBS1 interaction upon DNA damage (A) Coimmunoprecipitation shows reduced interaction between BRCA1-S1198A and SKP2 in HEK293 cells. (B) Recruitment of SKP2 upon microirradiation. SKP2 laser stripes (green) and gammaH2AX (red) at 10, 30, and 60 min time points are shown. Scale bar, 10 mum. Bar graph below is quantification of three independent experiments. For 10 min (n = 326 for Brca1 WT and n = 351 for Brca1 S1152A/S1152A , p = 0.007656); 30 min (n = 336 for Brca1 WT and n = 427 for Brca1 S1152A/S1152A , p = 0.009519); 60 min (n = 336 for Brca1 WT and n = 380 for Brca1 S1152A/S1152A , p = 0.009258). (C) Immunoprecipitation of endogenous NBS1 shows reduced K63-linked ubiquitination of NBS1 in Brca1 S1152A/S1152A MEF cells, but not in Brca1 S971A/S971A MEF cells, in response to DNA damage. (D) Coimmunoprecipitation shows reduced interaction between NBS1 and ATM upon DNA damage in Brca1 S1152A/S1152A MEF cells but not in Brca1 S971A/S971A MEF cells.