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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
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- Product number
- 82314 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATM Monoclonal Antibody (5C2)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: Raji, HeLa nuclear extract.
- Antibody clone number
- 5C2
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were lysed 72 hours after transfection. Cells were transfected with Transfection Reagent alone (Lane 1), 100nM ON-TARGETplus siCONTROL Non-Targeting Pool (Lane 2), or 100nM ONTARGETplus ATM siRNA (Lane 3). Western blot data for Lamin A/C is included as a control for equal protein loading.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were lysed 72 hours after transfection. Cells were transfected with Transfection Reagent alone (Lane 1), 100nM ON-TARGETplus siCONTROL Non-Targeting Pool (Lane 2), or 100nM ONTARGETplus ATM siRNA (Lane 3). Western blot data for Lamin A/C is included as a control for equal protein loading.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ATM was performed by separating 30 µg of HeLa whole cell and nuclear extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a ATM Monoclonal Antibody (5C2) (Product # 82314) at a dilution of 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ATM was performed by separating 30 µg of HeLa whole cell and nuclear extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a ATM Monoclonal Antibody (5C2) (Product # 82314) at a dilution of 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of ATM was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: ATM Monoclonal Antibody (5C2) (Product # 82314) diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
