MA1-46069

antibody from Invitrogen Antibodies

Targeting: ATM ATA, ATC, ATD, ATDC, TEL1, TELO1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-46069

Provider

Invitrogen Antibodies

Product name

Phospho-ATM (Ser1981) Monoclonal Antibody (10H11.E12)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

This antibody does not react with canine samples. Suggested positive control: Irradiated normal human fibroblasts. No reactivity against un-irradiated cell extracts.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

10H11.E12

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

ATM‑JAK‑PD‑L1 signaling pathway inhibition decreases EMT and metastasis of androgen‑independent prostate cancer.

Zhang L, Xu LJ, Zhu J, Li J, Xue BX, Gao J, Sun CY, Zang YC, Zhou YB, Yang DR, Shan YX
Molecular medicine reports 2018 May;17(5):7045-7054

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The Paradoxical Effects of Different Hepatitis C Viral Loads on Host DNA Damage and Repair Abilities.

Wang SC, Lai KR, Li CY, Chiang CS, Yu GY, Sakamoto N, Tu WY, Hsieh MH, Huang JF, Chuang WL, Dai CY, Yu ML
PloS one 2017;12(1):e0164281

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-ATM (Ser1981) using a monoclonal antibody (Product # MA1-46069).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-ATM (Ser1981) in irradiated or peroxidated human fibroblasts. Samples were incubated in Phospho-ATM (Ser1981) monoclonal antibody (Product # MA1-46069). Observed molecular weight 370 kDa. Theoretical molecular weight 351 kDa.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Phospho-ATM (Ser1981) in mouse spleen. Samples were incubated with Phospho-ATM (Ser1981) monoclonal antibody (Product # MA1-46069) followed by using DAB with hematoxylin counterstain.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1. (A) Reverse transcription-quantitative polymerase chain reaction and (B and C) immunohistochemistry analyses revealed enhanced p-ATM expression in prostate cancer tissues from patients with CRPC compared with those with HDPC. ***P

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2. In C4-2-ATMSi and CWR22Rv1-ATMSi knockout cell lines, migration and EMT are significantly suppressed compared with in the control group. (A) C4-2-ATMSi and CWR22Rv1-ATMSi cells were established by lentivirus infection, and decreased levels of p-ATM were demonstrated by reverse transcription-quantitative polymerase chain reaction and western blotting. (B) Migration ability of ATM knockout cells was significantly decreased compared with in the Sc cells, as revealed by a cell migration assay. Magnification, x40. (C) EMT of the ATM knockout cells was significantly decreased, as revealed by reverse transcription-quantitative polymerase chain reaction. **P

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig 4 The protein expression levels of HVL and LVL sorted cells by western blotting. (A) The ATM, phospho-ATM, GPX2, MRE11, OGG1, and XPC levels were measured by western blot analysis. The figure is representative of three independent experiments. (B) The relative fold activity was quantified using AlphaImage software and normalized to the expression of the internal actin control. * P < 0.05, ** P < 0.01.