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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [3]
- Immunoprecipitation [1]
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- Product number
- NB100-271 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-271, RRID:AB_10002501
- Product name
- Goat Polyclonal ATM Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references High-throughput genotoxicity assay identifies antioxidants as inducers of DNA damage response and cell death.
Involvement of DNA-dependent protein kinase in normal cell cycle progression through mitosis.
A genetic screen identifies the Triple T complex required for DNA damage signaling and ATM and ATR stability.
Inhibition of transforming growth factor-beta1 signaling attenuates ataxia telangiectasia mutated activity in response to genotoxic stress.
Fox JT, Sakamuru S, Huang R, Teneva N, Simmons SO, Xia M, Tice RR, Austin CP, Myung K
Proceedings of the National Academy of Sciences of the United States of America 2012 Apr 3;109(14):5423-8
Proceedings of the National Academy of Sciences of the United States of America 2012 Apr 3;109(14):5423-8
Involvement of DNA-dependent protein kinase in normal cell cycle progression through mitosis.
Lee KJ, Lin YF, Chou HY, Yajima H, Fattah KR, Lee SC, Chen BP
The Journal of biological chemistry 2011 Apr 8;286(14):12796-802
The Journal of biological chemistry 2011 Apr 8;286(14):12796-802
A genetic screen identifies the Triple T complex required for DNA damage signaling and ATM and ATR stability.
Hurov KE, Cotta-Ramusino C, Elledge SJ
Genes & development 2010 Sep 1;24(17):1939-50
Genes & development 2010 Sep 1;24(17):1939-50
Inhibition of transforming growth factor-beta1 signaling attenuates ataxia telangiectasia mutated activity in response to genotoxic stress.
Kirshner J, Jobling MF, Pajares MJ, Ravani SA, Glick AB, Lavin MJ, Koslov S, Shiloh Y, Barcellos-Hoff MH
Cancer research 2006 Nov 15;66(22):10861-9
Cancer research 2006 Nov 15;66(22):10861-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ATM Antibody [NB100-271] - Samples: Whole cell lysate (100 ug) from L-40 human (WT) or AT-59 human (AT) cells for WB. Whole cell lysate (0.5 mg) from L-40, AT-59, AT29 mouse (WT) or AP24 mouse (AT; ATM -/-) cells for IP. Antibody: Affinity purified goat anti-ATM NB100-271 used at 0.2 ug/ml for WB and 20 ug/mg lysate for IP. Detection: Chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ATM Antibody [NB100-271] - Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-ATM antibody NB100-271 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ATM Antibody [NB100-271] - Detection of human ATM by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat ATM antibody [NB100-271] used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Observed molecular weight ~360 kDa. Theoretical molecular weight 351 kDa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: ATM Antibody [NB100-271] - Detection of human ATM by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-ATM antibody NB100-271 used for IP at 3 ug per reaction. ATM was also immunoprecipitated by another goat anti-ATM antibody from Company B. For blotting immunoprecipitated ATM, NB100-271 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
