Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- PA5-49696 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BAG5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The antibody detects endogenous level of total BAG5 protein.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.3 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of BAG5 was performed by loading Lane1: A549 cell lysate (40µg), Lane2: Hela cell lysate (40µg), Lane3: 293T cell lysate (40µg) on to a 10% SDS-PAGE gel. Proteins were transferred to a membrane and the membrane was probed with a BAG5 antibody (Product # PA5-49696) at a 1/500 dilution for 1 minute, followed by Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution.