Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
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Validation data
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- Product number
- PA5-21434 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BAG5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, HeLa, Molt-4, Raji, mouse liver. Predicted reactivity: Mouse (92%), Rat (91%), Chicken (82%), Chimpanzee (99%), Bovine (93%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.68 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BAG5 was performed by separating 50 µg of mouse tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a BAG5 Polyclonal Antibody (Product # PA5-21434) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using BAG5 Polyclonal Antibody (Product # PA5-21434). Sample (30 µg of whole cell lysate). Lane A: Hela. Lane B: Molt-4. 10% SDS PAGE. BAG5 Polyclonal Antibody (Product # PA5-21434) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of BAG5 in paraformaldehyde-fixed HeLa cells using a BAG5 polyclonal antibody (Product # PA5-21434) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using BAG5 (Product # PA5-21434) antibody at 1:200 dilution.