PA5-51509
antibody from Invitrogen Antibodies
Targeting: ARHGAP4
C1, KIAA0131, p115, RhoGAP4, SrGAP4
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [8]
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Validation data
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- Product number
- PA5-51509 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RhoGAP4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: DRQTIETEEV NKTLKATLQA LLEVVASDDG DVLDSFQTSP STESLKSTSS DPGSRQAGRR RGQQQETETF YLTKLQEYLS GRSILAKLQA KHEKLQEALQ RGDKEEQEVS WTQYT Highest antigen sequence identity to the following orthologs: Mouse - 83%, Rat - 88%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.05 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of RhoGAP4 in human cell line U-2 OS shows positivity in cytoplasm, focal adhesion sites & nucleus but excluded from the nucleoli. Samples were probed using a RhoGAP4 Polyclonal Antibody (Product # PA5-51509).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RhoGAP4 in human spleen shows strong cytoplasmic positivity in cells in red pulp and cells in white pulp. Samples were probed using a RhoGAP4 Polyclonal Antibody (Product # PA5-51509).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RhoGAP4 in human spleen and cerebral cortex tissues using RhoGAP4 Polyclonal Antibody (Product # PA5-51509). Corresponding ARHGAP4 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RhoGAP4 in human colon, liver, lymph node and testis using RhoGAP4 Polyclonal Antibody (Product # PA5-51509) (A) shows similar protein distribution across tissues to an independent RhoGAP4 Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RhoGAP4 in human cerebral cortex using RhoGAP4 Polyclonal Antibody (Product # PA5-51509) shows low expression as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RhoGAP4 in human colon using RhoGAP4 Polyclonal Antibody (Product # PA5-51509).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RhoGAP4 in human testis using RhoGAP4 Polyclonal Antibody (Product # PA5-51509).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RhoGAP4 in human liver using RhoGAP4 Polyclonal Antibody (Product # PA5-51509).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RhoGAP4 in human lymph node using RhoGAP4 Polyclonal Antibody (Product # PA5-51509).