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antibody from Invitrogen Antibodies
Targeting: ARHGAP4
C1, KIAA0131, p115, RhoGAP4, SrGAP4
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [9]
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- Product number
- PA5-51499 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RhoGAP4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: EHNGMRGLIP HKYITLPAGT EKQVVGAGLQ TAGESGSSPE GLLASELVHR PEPCTSPEAM GPSGHRRRCL VPASPEQHVE VDKAVAQNMD SVFKELLGKT SVRQGLGPAS TTSP Highest antigen sequence identity to the following orthologs: Mouse - 70%, Rat - 72%.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RhoGAP4 in human cell line RPMI-8226 using a RhoGAP4 Polyclonal Antibody (Product # PA5-51499).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RhoGAP4 in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12 using a RhoGAP4 Polyclonal Antibody (Product # PA5-51499).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RhoGAP4 in human cerebral cortex using RhoGAP4 Polyclonal Antibody (Product # PA5-51499) shows low expression as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RhoGAP4 in human testis using RhoGAP4 Polyclonal Antibody (Product # PA5-51499).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RhoGAP4 in human spleen and cerebral cortex tissues using RhoGAP4 Polyclonal Antibody (Product # PA5-51499). Corresponding ARHGAP4 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RhoGAP4 in human colon, liver, lymph node and testis using RhoGAP4 Polyclonal Antibody (Product # PA5-51499) (A) shows similar protein distribution across tissues to an independent RhoGAP4 Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RhoGAP4 in human cerebral cortex using RhoGAP4 Polyclonal Antibody (Product # PA5-51499) shows low expression as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RhoGAP4 in human spleen using RhoGAP4 Polyclonal Antibody (Product # PA5-51499) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RhoGAP4 in human liver using RhoGAP4 Polyclonal Antibody (Product # PA5-51499).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RhoGAP4 in human colon using RhoGAP4 Polyclonal Antibody (Product # PA5-51499).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RhoGAP4 in human lymph node using RhoGAP4 Polyclonal Antibody (Product # PA5-51499).
