PA5-64730

antibody from Invitrogen Antibodies

Targeting: XPA XP1, XPAC

Antibody data

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Validation data

Product number: PA5-64730 - Provider product page
Provider: Invitrogen Antibodies
Product name: Phospho-XPA (Ser196) Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: Phospho-XPA (Ser196) Polyclonal Antibody detects endogenous levels of XPA only when phosphorylated at Ser196.
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: -20°C

XPA protein structure - PA5-64730 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4 beta-HPV 8E6 attenuates CHK1 phosphorylation. ( A ) Representative immunoblots of untreated hTERT HFKs with vector control (LXSN) and beta-HPV 8E6 cell lines. Nucleolin was used as a loading control. ( B ) mRNA expression level of CHEK1 in vector control (LXSN) and beta-HPV 8E6 expressing primary HFKs as measured by RT-qPCR and normalized towards the expression level of beta-actin. Data shown in figures are the means of +-SE of three independent experiments. ( C ) Representative immunoblots of hTERT HFKs with vector control (LXSN) and beta-HPV 8E6 harvested 0-6 h post 5mJ/cm 2 UVR. Nucleolin was used as a loading control. ( D ) Representative immunoblots of primary HFKs with vector control (LXSN) and beta-HPV 8E6 harvested 0-8 h post 5 mJ/cm 2 UVR. Nucleolin was used as a loading control. ( E ) Representative immunoblots of hTERT HFKs with vector control (LXSN) and beta-HPV 8E6 harvested 0-8 h post 5 mJ/cm 2 UVR. Nucleolin was used as a loading control. ( A, C - E ) The numbers above bands represent quantification by densitometry. This is shown relative to untreated cells within the same cell line and normalized to the loading control. ( F ) Cell cycle analysis of hTERT HFKs with LXSN vector control and beta-HPV 8E6 1 h post 5 mJ/cm 2 UVR.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 5 beta-HPV 8E6 attenuates XPA phosphorylation. ( A ) Representative immunoblots of untreated hTERT HFKs with vector control (LXSN) and beta-HPV 8E6 cell lines. Nucleolin was used as a loading control. ( B ) mRNA expression level of XPA in vector control (LXSN) and beta-HPV 8E6 expressing primary HFKs as measured by RT-qPCR and normalized towards the expression level of beta-actin. Data shown in figures are the means of +-SE of three independent experiments. ( C ) Representative immunoblots of hTERT HFKs with vector control (LXSN) and beta-HPV 8E6 harvested 0-6 h post 5 mJ/cm 2 UVR. Nucleolin was used as a loading control. ( D ) Representative immunoblots of primary HFKs with vector control (LXSN) and beta-HPV 8E6 harvested 0-8 h post 5 mJ/cm 2 UVR. Nucleolin was used as a loading control. ( A , C , D ) The numbers above bands represent quantification by densitometry. This is shown relative to untreated cells within the same cell line and normalized to the loading control. ( E ) Subcellular fractionation of hTERT HFKs with vector control (LXSN) and beta-HPV 8E6 cell line lysates harvested 6 h post exposure to 5 mJ/cm 2 UVR were observed via immunoblot. GAPDH was used as a cytoplasmic loading control and Nucleolin was used as a nuclear loading control.