- MA5-13835 - Invitrogen Antibodies XPA antibody

Submitted references Elevated glucose increases genomic instability by inhibiting nucleotide excision repair.

Ciminera AK, Shuck SC, Termini J
Life science alliance 2021 Oct;4(10)

Progerin sequestration of PCNA promotes replication fork collapse and mislocalization of XPA in laminopathy-related progeroid syndromes.

Hilton BA, Liu J, Cartwright BM, Liu Y, Breitman M, Wang Y, Jones R, Tang H, Rusinol A, Musich PR, Zou Y
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2017 Sep;31(9):3882-3893

A rapid assay for measuring nucleotide excision repair by oligonucleotide retrieval.

Shen JC, Fox EJ, Ahn EH, Loeb LA
Scientific reports 2014 May 8;4:4894

ATR pathway inhibition is synthetically lethal in cancer cells with ERCC1 deficiency.

Mohni KN, Kavanaugh GM, Cortez D
Cancer research 2014 May 15;74(10):2835-45

Physiological modulation of endogenous BRCA1 p220 abundance suppresses DNA damage during the cell cycle.

Dimitrov SD, Lu D, Naetar N, Hu Y, Pathania S, Kanellopoulou C, Livingston DM
Genes & development 2013 Oct 15;27(20):2274-91

Lack of CAK complex accumulation at DNA damage sites in XP-B and XP-B/CS fibroblasts reveals differential regulation of CAK anchoring to core TFIIH by XPB and XPD helicases during nucleotide excision repair.

Zhu Q, Wani G, Sharma N, Wani A
DNA repair 2012 Dec 1;11(12):942-50

GCN5 and E2F1 stimulate nucleotide excision repair by promoting H3K9 acetylation at sites of damage.

Guo R, Chen J, Mitchell DL, Johnson DG
Nucleic acids research 2011 Mar;39(4):1390-7

Sodium arsenite and hyperthermia modulate cisplatin-DNA damage responses and enhance platinum accumulation in murine metastatic ovarian cancer xenograft after hyperthermic intraperitoneal chemotherapy (HIPEC).

Muenyi CS, States VA, Masters JH, Fan TW, Helm CW, States JC
Journal of ovarian research 2011 Jun 22;4:9

Dissociation of CAK from core TFIIH reveals a functional link between XP-G/CS and the TFIIH disassembly state.

Arab HH, Wani G, Ray A, Shah ZI, Zhu Q, Wani AA
PloS one 2010 Jun 8;5(6):e11007

Chromatin restoration following nucleotide excision repair involves the incorporation of ubiquitinated H2A at damaged genomic sites.

Zhu Q, Wani G, Arab HH, El-Mahdy MA, Ray A, Wani AA
DNA repair 2009 Feb 1;8(2):262-73

Recognition of cisplatin-DNA interstrand cross-links by replication protein A.

Patrick SM, Tillison K, Horn JM
Biochemistry 2008 Sep 23;47(38):10188-96

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DNA polymerase epsilon associates with the elongating form of RNA polymerase II and nascent transcripts.

Rytkönen AK, Hillukkala T, Vaara M, Sokka M, Jokela M, Sormunen R, Nasheuer HP, Nethanel T, Kaufmann G, Pospiech H, Syväoja JE
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Cancer 2005 Jun 1;103(11):2313-9

Xeroderma pigmentosum group a protein and chemotherapy resistance in human germ cell tumors.

Honecker F, Mayer F, Stoop H, Oosterhuis JW, Koch S, Bokemeyer C, Looijenga LH
Laboratory investigation; a journal of technical methods and pathology 2003 Oct;83(10):1489-95

Functional implications of antiestrogen induction of quinone reductase: inhibition of estrogen-induced deoxyribonucleic acid damage.

Bianco NR, Perry G, Smith MA, Templeton DJ, Montano MM
Molecular endocrinology (Baltimore, Md.) 2003 Jul;17(7):1344-55

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot of XPA using XPA Monoclonal Antibody (Product # MA5-13835) on LS174T Cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of XPA was achieved by transfecting A431 cells with XPA specific siRNAs (Silencer® select Product # s14926 and s14927). Western blot analysis (Fig a) was performed using Modified whole cell extract (1% SDS) from the XPA knock down cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with Anti-XPA Mouse Monoclonal Antibody (Product # MA5-13835, 1 µg/mL) and Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig b). Loss of signal upon siRNA mediated knock down confirms that antibody is specific to XPA.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of A-431 (Lane 1), Jurkat (Lane 2), HeLa (Lane 3), MCF7 (Lane 4), K-562 (Lane 5), Hep G2 (Lane 6), Raji (Lane 7), HCT 116 (Lane 8), and PANC-1 (Lane 9). The blots were probed with Mouse Anti-XPA Monoclonal Antibody (Product # MA5-13835, 1 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 37 kDa band corresponding to XPA was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of XPA was performed using 70 % confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with XPA (12F5) Mouse Monoclonal antibody (Product # MA5-13835) at 5 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Formalin-fixed, paraffin-embedded human tonsil stained with XPA antibody using peroxidase-conjugate and AEC chromogen. Note nuclear staining of epithelial cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 7. Nucleotide excision repair (NER) gene expression and function are regulated by HIF-1alpha. (A) 293T WT HG cells were treated with 50 muM daprodustat for 0-24 h to stabilize HIF-1alpha. NER genes were monitored via qRT-PCR (n = 2). (B) 293T WT cells in HG were treated with 100 muM CoCl 2 for 6 h. HIF-1alpha, XPD, XPA, and VEGFA proteins were detected by Western blot and quantified by densitometry, normalized to beta-actin. (C) qRT-PCR measurement of NER gene expression in cells treated for 6 h with DMSO (vehicle) or 0.1 muM echinomycin, an inhibitor of HIF-1alpha binding to HREs (n = 3). (D) 293T WT cells in LG or HG were treated with increasing doses of echinomycin and XPA protein was assessed by Western blot. (E) 293T HG cells were transfected with CEdG-modified pM1-luc (366 CEdG/10 5 dG) and treated with CoCl 2 6 h before measuring luminescence (paired t test). (F) WT cells in HG and XPC cells in LG were treated with CoCl 2 for 24 h before measurement of CEdG in genomic DNA by LC-MS/MS (one-way ANOVA with Tukey's multiple comparisons, technical triplicate).

MA5-13835

Antibody data