Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-37775 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-p130Cas (Tyr410) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control for Western blot is K562 or NIH-3T3 cells; suggested positive control for IHC is human breast carcinoma.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Src inhibition attenuates polyglutamine-mediated neuromuscular degeneration in spinal and bulbar muscular atrophy.
Iida M, Sahashi K, Kondo N, Nakatsuji H, Tohnai G, Tsutsumi Y, Noda S, Murakami A, Onodera K, Okada Y, Nakatochi M, Tsukagoshi Okabe Y, Shimizu S, Mizuno M, Adachi H, Okano H, Sobue G, Katsuno M
Nature communications 2019 Sep 19;10(1):4262
Nature communications 2019 Sep 19;10(1):4262
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from K562 cells (Lane 2) and 3T3 cells (Lane 3) using P130 Cas (pTyr410) polyclonal antibody (Product # PA5-37775) compared to the same antibody preincubated with a blocking peptide (Lane 1).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Phospho-p130Cas (Tyr410) in paraffin-embedded Human breast carcinoma tissue using Phospho-p130Cas (Tyr410) Polyclonal Antibody (Product # PA5-37775) (left) or the same antibody preincubated with blocking peptide (right).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 7 p130Cas is activated in cellular and mouse models of SBMA. a , b Immunoblots showing the levels of the phosphorylated and total p130Cas proteins in NSC34 cells and C2C12 cells stably expressing AR-24Q or AR-97Q that were treated with or without DHT ( a ). Quantitative analyses were performed using densitometry ( n = 3) ( b ). c - e Immunoblots showing levels of the p-p130Cas and p130Cas proteins in the spinal cord and skeletal muscle of wild-type and AR-97Q mice at 6, 9 and 13 weeks of age ( c ). Quantitative analyses were performed using densitometry ( n = 3) ( d , e ). f , g Immunohistochemistry for p-p130Cas in spinal cord ( f ) and skeletal muscle ( g ) samples from 6-week-old mice. Error bars indicate the s.e.m. Statistical analyses were performed using the unpaired two-sided t -test ( b , d , e ). * p < 0.05 and ** p < 0.01. Wt, wild-type; AR, AR-97Q; N.S., not significant. Scale bars: 25 um ( f, g ). Source data are provided as a Source Data file ( a - e )