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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [3]
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- Product number
- TA502536 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA502536, RRID:AB_11124646
- Product name
- BCAR1 mouse monoclonal antibody, clone OTI3A11 (formerly 3A11)
- Antibody type
- Monoclonal
- Description
- BCAR1 mouse monoclonal antibody, clone OTI3A11 (formerly 3A11)
- Reactivity
- Canine
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- BCAR1
- Isotype
- IgG
- Antibody clone number
- OTI3A11
- Vial size
- 100 µl
- Concentration
- 0.66 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BCAR1 monoclonal antibody.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCAR1 (RC209133, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCAR1.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-BCAR1 mouse monoclonal antibody (TA502536) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BCAR1(RC209133).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-BCAR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502536)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-BCAR1 antibody(TA502536),(Red), compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Hela cells, using anti-BCAR1 antibody(TA502536),(Red), compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC209133 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-BCAR1 antibody(TA502536), and then analyzed by flow cytometry.
- Validation comment
- FC
