AF5779
antibody from Novus Biologicals
Targeting: CDKN2A
ARF, CDK4I, CDKN2, CMM2, INK4, INK4a, MLM, MTS1, p14, p14ARF, p16, p16INK4a, p19, p19Arf
Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF5779 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal p16INK4a/CDKN2A Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human p16INK4a/CDKN2A in Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Modeling Progressive Fibrosis with Pluripotent Stem Cells Identifies an Anti-fibrotic Small Molecule.
Exposure of human melanocytes to UVB twice and subsequent incubation leads to cellular senescence and senescence-associated pigmentation through the prolonged p53 expression.
MAX inactivation is an early event in GIST development that regulates p16 and cell proliferation.
Adiponectin corrects premature cellular senescence and normalizes antimicrobial peptide levels in senescent keratinocytes.
Induction of heparanase by HPV E6 oncogene in head and neck squamous cell carcinoma.
Vijayaraj P, Minasyan A, Durra A, Karumbayaram S, Mehrabi M, Aros CJ, Ahadome SD, Shia DW, Chung K, Sandlin JM, Darmawan KF, Bhatt KV, Manze CC, Paul MK, Wilkinson DC, Yan W, Clark AT, Rickabaugh TM, Wallace WD, Graeber TG, Damoiseaux R, Gomperts BN
Cell reports 2019 Dec 10;29(11):3488-3505.e9
Cell reports 2019 Dec 10;29(11):3488-3505.e9
Exposure of human melanocytes to UVB twice and subsequent incubation leads to cellular senescence and senescence-associated pigmentation through the prolonged p53 expression.
Choi SY, Bin BH, Kim W, Lee E, Lee TR, Cho EG
Journal of dermatological science 2018 Jun;90(3):303-312
Journal of dermatological science 2018 Jun;90(3):303-312
MAX inactivation is an early event in GIST development that regulates p16 and cell proliferation.
Schaefer IM, Wang Y, Liang CW, Bahri N, Quattrone A, Doyle L, Mariño-Enríquez A, Lauria A, Zhu M, Debiec-Rychter M, Grunewald S, Hechtman JF, Dufresne A, Antonescu CR, Beadling C, Sicinska ET, van de Rijn M, Demetri GD, Ladanyi M, Corless CL, Heinrich MC, Raut CP, Bauer S, Fletcher JA
Nature communications 2017 Mar 8;8:14674
Nature communications 2017 Mar 8;8:14674
Adiponectin corrects premature cellular senescence and normalizes antimicrobial peptide levels in senescent keratinocytes.
Jin T, Kim MJ, Heo WI, Park KY, Choi SY, Lee MK, Hong SP, Kim SJ, Im M, Moon NJ, Seo SJ
Biochemical and biophysical research communications 2016 Sep 2;477(4):678-684
Biochemical and biophysical research communications 2016 Sep 2;477(4):678-684
Induction of heparanase by HPV E6 oncogene in head and neck squamous cell carcinoma.
Hirshoren N, Bulvik R, Neuman T, Rubinstein AM, Meirovitz A, Elkin M
Journal of cellular and molecular medicine 2014 Jan;18(1):181-6
Journal of cellular and molecular medicine 2014 Jan;18(1):181-6
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human p16INK4a/CDKN2A by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line, HepG2 human hepatocellular carcinoma cell line, and Saos-2 human osteosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human p16INK4a/CDKN2A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5779) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for p16INK4a/CDKN2A at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human p16INK4a/ CDKN2A by Simple WesternTM. Simple Western lane view shows lysates of HEK293 human embryonic kidney cell line, loaded at 0.2 mg/mL. A specific band was detected for p16INK4a/ CDKN2A at approximately 24 kDa (as indicated) using 10 µg/mL of Goat Anti-Human p16INK4a/ CDKN2A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5779) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.