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LearnTA805237
antibody from OriGene
Targeting: CDKN2A
ARF, CDK4I, CDKN2, CMM2, INK4, INK4a, MLM, MTS1, p14, p14ARF, p16, p16INK4a, p19, p19Arf
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- TA805237 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA805237, RRID:AB_2627640
- Product name
- CDKN2A (p16INK4a) mouse monoclonal antibody, clone OTI5H5 (formerly 5H5)
- Antibody type
- Monoclonal
- Description
- CDKN2A (p16INK4a) mouse monoclonal antibody, clone OTI5H5 (formerly 5H5)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- CDKN2A
- Isotype
- IgG
- Antibody clone number
- OTI5H5
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN2A (RC220937, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDKN2A.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-CDKN2A monoclonal antibody at 1:200.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CDKN2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805237)
- Validation comment
- IHC
