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LearnLS-C2743
antibody from LSBio
Targeting: CDKN2A
ARF, CDK4I, CDKN2, CMM2, INK4, INK4a, MLM, MTS1, p14, p14ARF, p16, p16INK4a, p19, p19Arf
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunoprecipitation [1]
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Validation data
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- Product number
- LS-C2743 - Provider product page
- Provider
- LSBio
- Product name
- CDKN2A / p16INK4a Antibody (aa54-75, clone 12A11) LS-C2743
- Antibody type
- Monoclonal
- Reactivity
- Mouse
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- 12A11
- Storage
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
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Supportive validation
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- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitation of p19Arf using LS-C2743. Total cell lysates (250 ug) from NIH3T3 cells which have deleted the Arf gene (lane 5) and from NIH3T3 cells expressing an HA-tagged version of p19Arf (lanes 3, 4) were incubated with control rat IgG (lane 3), or LS-C2743 (lanes 4, 5) and protein G- Sepharose. Precipitated proteins were resolved by SDS-PAGE and analyzed by Western blotting using an anti-HA antibody. Unprecipitated total cell lysates (25 ug, equivalent to 10% of immunoprecipitation) f.
