Explore
Validate
LearnMAB12264
antibody from Abnova Corporation
Targeting: CDKN2A
ARF, CDK4I, CDKN2, CMM2, INK4, INK4a, MLM, MTS1, p14, p14ARF, p16, p16INK4a, p19, p19Arf
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB12264 - Provider product page
- Provider
- Abnova Corporation
- Product name
- CDKN2A monoclonal antibody, clone 1D7D2A1
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant human CDKN2A.
- Isotype
- IgG
- Antibody clone number
- 1D7D2A1
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of human CDKN2A (AA: 1-156) recombinant protein (Expected MW is 19 kDa) with CDKN2A monoclonal antibody, clone 1D7D2A1 (Cat# MAB12264) at 1:500-1:2000 dilution.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [CDKN2A (AA: 1-156)-hIgGFc transfected HEK293 cells] with CDKN2A monoclonal antibody, clone 1D7D2A1 (Cat# MAB12264) at 1:500-1:2000 dilution.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of Lane 1: Hela cell lysate; Lane 2: HepG2 cell lysate; Lane 3: Hek293 cell lysate with CDKN2A monoclonal antibody, clone 1D7D2A1 (Cat# MAB12264) at 1:500-1:2000 dilution.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ELISA analysis of CDKN2A monoclonal antibody, clone 1D7D2A1 (Cat# MAB12264) at 1:10000 dilution.
- Validation comment
- ELISA
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human endometrial cancer tissues (A) and esophageal cencer tissues (B) with CDKN2A monoclonal antibody, clone 1D7D2A1 (Cat# MAB12264) at 1:200-1:1000 dilution.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of Hela cells with CDKN2A monoclonal antibody, clone 1D7D2A1 (Cat# MAB12264) at 1:200-1:400 dilution (Green) and negative control (Red).
- Validation comment
- Flow Cytometry
