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LearnNB200-169
antibody from Novus Biologicals
Targeting: CDKN2A
ARF, CDK4I, CDKN2, CMM2, INK4, INK4a, MLM, MTS1, p14, p14ARF, p16, p16INK4a, p19, p19Arf
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
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- Product number
- NB200-169 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB200-169, RRID:AB_10003248
- Product name
- p19ARF Antibody
- Antibody type
- Monoclonal
- Antigen
- A synthetic peptide containing amino acids within 1-100 of the murine p19ARF.
- Reactivity
- Human, Mouse, Hamster
- Host
- Rat
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Cell-nonautonomous local and systemic responses to cell arrest enable long-bone catch-up growth in developing mice.
RABL6A, a novel RAB-like protein, controls centrosome amplification and chromosome instability in primary fibroblasts.
A non-tumor suppressor role for basal p19ARF in maintaining nucleolar structure and function.
The AKT-mTOR pathway plays a critical role in the development of leiomyosarcomas.
Increased DNA damage sensitivity and apoptosis in cells lacking the Snf5/Ini1 subunit of the SWI/SNF chromatin remodeling complex.
Roselló-Díez A, Madisen L, Bastide S, Zeng H, Joyner AL
PLoS biology 2018 Jun;16(6):e2005086
PLoS biology 2018 Jun;16(6):e2005086
RABL6A, a novel RAB-like protein, controls centrosome amplification and chromosome instability in primary fibroblasts.
Zhang X, Hagen J, Muniz VP, Smith T, Coombs GS, Eischen CM, Mackie DI, Roman DL, Van Rheeden R, Darbro B, Tompkins VS, Quelle DE
PloS one 2013;8(11):e80228
PloS one 2013;8(11):e80228
A non-tumor suppressor role for basal p19ARF in maintaining nucleolar structure and function.
Apicelli AJ, Maggi LB Jr, Hirbe AC, Miceli AP, Olanich ME, Schulte-Winkeler CL, Saporita AJ, Kuchenreuther M, Sanchez J, Weilbaecher K, Weber JD
Molecular and cellular biology 2008 Feb;28(3):1068-80
Molecular and cellular biology 2008 Feb;28(3):1068-80
The AKT-mTOR pathway plays a critical role in the development of leiomyosarcomas.
Hernando E, Charytonowicz E, Dudas ME, Menendez S, Matushansky I, Mills J, Socci ND, Behrendt N, Ma L, Maki RG, Pandolfi PP, Cordon-Cardo C
Nature medicine 2007 Jun;13(6):748-53
Nature medicine 2007 Jun;13(6):748-53
Increased DNA damage sensitivity and apoptosis in cells lacking the Snf5/Ini1 subunit of the SWI/SNF chromatin remodeling complex.
Klochendler-Yeivin A, Picarsky E, Yaniv M
Molecular and cellular biology 2006 Apr;26(7):2661-74
Molecular and cellular biology 2006 Apr;26(7):2661-74
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: p19ARF/CDKN2A Antibody (12-A1-1) [NB200-169] - Analysis of endogenous p19Arf using NB200-169. Total cell lysates (25 ug) from NIH3T3 cells, which have deleted the Arf gene (Lanes 1) and from wild type mouse embryo fibroblasts (MEFs) at passage 6, which express p19Arf (Lanes 2), were resolved by SDS-PAGE. The membrane was probed with 1ug/ml purified p19Arf (NB200-169).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: p19ARF/CDKN2A Antibody (12-A1-1) [NB200-169] - Total cell lysates (250 ug) from NIH3T3 cells which have deleted the Arf gene (Lanes 5) and from NIH3T3 cells expressing an HA-tagged version of p19Arf (lanes 3, 4) were incubated with control rat IgG (Lanes 3), or NB200-169 (lanes 4, 5) and protein G- Sepharose. Precipitated proteins were resolved by SDS-PAGE and analysed by Western blotting using an anti-HA antibody. Unprecipitated total cell lysates (25 ug, equivalent to 10% of immunoprecipitation) from Arf null NIH3T3 cells (Lanes 1) and HA- p19Arf expressing cells (Lanes 2) were also analyzed by Western blotting, as input controls.
