PA1-16639

antibody from Invitrogen Antibodies

Targeting: CDKN2A ARF, CDK4I, CDKN2, CMM2, INK4, INK4a, MLM, MTS1, p14, p14ARF, p16, p16INK4a, p19, p19Arf

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-16639

Provider

Invitrogen Antibodies

Product name

p14ARF Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

p14ARF tends to run slightly higher than the theoretical MW of 14 kDa. Suggested positive control: BT549 cells or HeLa whole cell extract, antigen standard for CDKN2A (transient overexpression lysate).

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C or -80°C if preferred

References

Submitted references

Molecular Consequences of Depression Treatment: A Potential In Vitro Mechanism for Antidepressants-Induced Reprotoxic Side Effects.

Sołek P, Mytych J, Tabęcka-Łonczyńska A, Koziorowski M
International journal of molecular sciences 2021 Nov 1;22(21)

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Protective role of klotho protein on epithelial cells upon co-culture with activated or senescent monocytes.

Mytych J, Wos I, Solek P, Koziorowski M
Experimental cell research 2017 Jan 15;350(2):358-367

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of p14ARF Antibody in HeLa whole cell lysate (lot C).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of p14ARF in HeLa whole Cell lysate. Samples were incubated in p14ARF polyclonal antibody (Product # PA1-16639). ECL detection method.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of p14ARF in HeLa cells. Samples were incubated in p14ARF polyclonal antibody (Product # PA1-16639) followed by DyLight 488 (green). Nuclei were counterstained with DAPI (blue). Tubulin was stained with alpha tubulin (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of p14ARF Antibody in HeLa cells. Panel one is a brightfield image, panel two shows the immunofluorescence staining of p14RF detected using Dylight 488 and panel three is an overlay of showing the nuclear localization of the p14ARF staining.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of p14ARF in HeLa cells. Samples were incubated in p14ARF polyclonal antibody (Product # PA1-16639) using a dilution of 1:400. Alexa Fluor 488 secondary (shown in purple). M1 is defined by unstained cells.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 Antidepressant-induced cell cycle profile regulation through related genes activation. GC-1 spg and GC-2 spd cells were treated with antidepressants for 48 and 96 h, the cell cycle profile ( A , B , D , E ) was determined and the level of p16, p21, p27, p53 proteins was controlled by Western blot technique. Representative blots are shown ( C , F ). Statistical differences were determined using one-way analysis of variance (ANOVA) with Dunnett's post-hoc test; p values < 0.05 were considered statistically significant. Asterisks (*) indicate the comparison between control and antidepressants-treated cells, whereas carets (^) indicate the comparison between the same drugs in different periods (48 vs. 96). Bars indicate mean +- SD, n = 3, ***/^^^ p < 0.001, **/^^ p < 0.01, */^ p < 0.05, no indication-no statistical significance.