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antibody from Invitrogen Antibodies
Targeting: CDKN2A
ARF, CDK4I, CDKN2, CMM2, INK4, INK4a, MLM, MTS1, p14, p14ARF, p16, p16INK4a, p19, p19Arf
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- PA1-16639 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- p14ARF Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- p14ARF tends to run slightly higher than the theoretical MW of 14 kDa. Suggested positive control: BT549 cells or HeLa whole cell extract, antigen standard for CDKN2A (transient overexpression lysate).
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C or -80°C if preferred
Submitted references Molecular Consequences of Depression Treatment: A Potential In Vitro Mechanism for Antidepressants-Induced Reprotoxic Side Effects.
Protective role of klotho protein on epithelial cells upon co-culture with activated or senescent monocytes.
Sołek P, Mytych J, Tabęcka-Łonczyńska A, Koziorowski M
International journal of molecular sciences 2021 Nov 1;22(21)
International journal of molecular sciences 2021 Nov 1;22(21)
Protective role of klotho protein on epithelial cells upon co-culture with activated or senescent monocytes.
Mytych J, Wos I, Solek P, Koziorowski M
Experimental cell research 2017 Jan 15;350(2):358-367
Experimental cell research 2017 Jan 15;350(2):358-367
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of p14ARF Antibody in HeLa whole cell lysate (lot C).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of p14ARF in HeLa whole Cell lysate. Samples were incubated in p14ARF polyclonal antibody (Product # PA1-16639). ECL detection method.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of p14ARF in HeLa cells. Samples were incubated in p14ARF polyclonal antibody (Product # PA1-16639) followed by DyLight 488 (green). Nuclei were counterstained with DAPI (blue). Tubulin was stained with alpha tubulin (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of p14ARF in HeLa cells. Samples were incubated in p14ARF polyclonal antibody (Product # PA1-16639) using a dilution of 1:400. Alexa Fluor 488 secondary (shown in purple). M1 is defined by unstained cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Antidepressant-induced cell cycle profile regulation through related genes activation. GC-1 spg and GC-2 spd cells were treated with antidepressants for 48 and 96 h, the cell cycle profile ( A , B , D , E ) was determined and the level of p16, p21, p27, p53 proteins was controlled by Western blot technique. Representative blots are shown ( C , F ). Statistical differences were determined using one-way analysis of variance (ANOVA) with Dunnett's post-hoc test; p values < 0.05 were considered statistically significant. Asterisks (*) indicate the comparison between control and antidepressants-treated cells, whereas carets (^) indicate the comparison between the same drugs in different periods (48 vs. 96). Bars indicate mean +- SD, n = 3, ***/^^^ p < 0.001, **/^^ p < 0.01, */^ p < 0.05, no indication-no statistical significance.
