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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- H00006693-B01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00006693-B01, RRID:AB_1017513
- Product name
- SPN MaxPab mouse polyclonal antibody (B01)
- Antibody type
- Polyclonal
- Description
- Mouse polyclonal antibody raised against a full-length human SPN protein.
- Antigen sequence
MATLLLLLGVLVVSPDALGSTTAVQTPTSGEPLVS
TSEPLSSKMYTTSITSDPKADSTGDQTSALPPSTS
INEGSPLWTSIGASTGSPLPEPTTYQEVSIKMSSV
PQETPHATSHPAVPITANSLGSHTVTGGTITTNSP
ETSSRTSGAPVTTAASSLETSRGTSGPPLTMATVS
LETSKGTSGPPVTMATDSLETSTGTTGPPVTMTTG
SLEPSSGASGPQVSSVKLSTMMSPTTSTNASTVPF
RNPDENSRGMLPVAVLVALLAVIVLVALLLLWRRR
QKRRTGALVLSRGGKRNGVVDAWAGPAQVPEEGAV
TVTVGGSGGDKGSGFPDGEGSSRRPTLTTFFGRRK
SRQGSLAMEELKSGSGPSLKGEEEPLVASEDGAVD
APAPDEPEGGDGAAP- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of SPN expression in transfected 293T cell line (H00006693-T01) by SPN MaxPab polyclonal antibody.Lane 1: SPN transfected lysate(44 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of purified MaxPab antibody to SPN on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- FACS analysis of negative control 293 cells (Black) and SPN expressing 293 cells (Green) using SPN purified MaxPab mouse polyclonal antibody.
- Validation comment
- Flow Cytometry
