Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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Validation data
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- Product number
- V3651 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- CD43 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A affinity chromatography
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antibody clone number
- SPN/2049R
- Vial size
- 20 µg, 100 µg (with BSA, sodium azide), 100 µg (without BSA, sodium azide), 7 ml (prediluted for IHC)
- Concentration
- 0.2 mg/ml, 1 mg/ml (BSA/azide free format), Prediluted (IHC only format)
- Storage
- Store the recombinant CD43 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human K562 cell lysate with recombinant CD43 antibody (clone SPN/2049R). Expected molecular weight 45-115 kDa depending on glycosylation level.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescence staining of human K562 cells with recombinant CD43 antibody (green, clone SPN/2049R) and NucSpot (red).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human tonsil with recombinant CD43 antibody (clone SPN/2049R). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- FACS staining of PFA-fixed human K562 cells with recombinant CD43 antibody (clone SPN/2049R). Red=isotype control, Blue= recombinant CD43 antibody.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE analysis of purified, BSA-free recombinant CD43 antibody (clone SPN/2049R) as confirmation of integrity and purity.