MA5-12194

antibody from Invitrogen Antibodies

Targeting: SPN CD43, GPL115, LSN

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-12194

Provider

Invitrogen Antibodies

Product name

CD43 Monoclonal Antibody (DF-T1)

Antibody type

Monoclonal

Antigen

Other

Description

MA5-12194 targets CD43 in FACS, WB and IHC (P) applications and shows reactivity with Human samples. The MA5-12194 immunogen is myeloblastic KG1 cells.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

DF-T1

Vial size

500 µL

Concentration

0.2 mg/mL

Storage

4° C

References

Submitted references

Human Immunodeficiency Virus (HIV)-Negative and Human Herpes Virus-8 (HHV-8)-Positive Primary Effusion Lymphoma: A Case Report and Review of the Literature.

Güven Karataş S, Bayrak R, Sahin Balçık O, Yalçın KS, Atıcı E, Akyıldız U, Koşar A
Turkish journal of haematology : official journal of Turkish Society of Haematology 2013 Mar;30(1):67-71

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Primary cutaneous histiocyte and neutrophil-rich CD30+ and CD56+ anaplastic large-cell lymphoma with prominent angioinvasion and nerve involvement in the forehead and scalp of an immunocompetent woman.

Boudova L, Kazakov DV, Jindra P, Sima R, Vanecek T, Kuntscher V, Vera V, Bouda J, Michal M
Journal of cutaneous pathology 2006 Aug;33(8):584-9

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Haptoglobin interacts with the human mast cell line HMC-1 and inhibits its spontaneous proliferation.

El-Ghmati SM, Arredouani M, Van Hoeyveld EM, Ceuppens JL, Stevens EA
Scandinavian journal of immunology 2002 Apr;55(4):352-8

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CD43 was performed by loading 25 µg of CEM cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a CD43 monoclonal antibody (Product # MA5-12194) at a dilution of 1:50 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~95-135 kDa.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Formalin-fixed, paraffin-embedded human tonsil stained with CD43 antibody using peroxidase-conjugate and DAB chromogen. Note cell membrane staining of T lymphocytes.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of CD43 in PBMC cells (green) compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 µL of cell solution was added to each tube at a dilution of 2x10^7 cells/mL, followed by the addition of 50 µL of isotype control and primary antibody (Product # MA5-12194) at a dilution of 0.5 µg/test. Cells were incubated for 30 min at 4ºC and washed with a cell buffer, followed by incubation with a DyLight 488-conjugated secondary antibody for 30 min at 4ºC in the dark. FACS analysis was performed using 400 µL of cell buffer.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of CD43 in Ramos cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a CD43 monoclonal antibody (Product # MA5-12194) at a dilution of 0.5 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.