LS-C375596

antibody from LSBio

Targeting: DFFA DFF-45, DFF1, DFF45, ICAD

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C375596

Provider

LSBio

Product name

DFFA / ICAD / DFF45 Antibody (aa1-331) LS-C375596

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot. All lanes: DFFA antibody at 10 ug/ml. Lane 1: HeLa whole cell lysate. Lane 2: 293T whole cell lysate. Lane 3: PC-3 whole cell lysate. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 37 kDa. Observed band size: 37 kDa.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitating DFFA in Hela whole cell lysate Lane 1: Rabbit monoclonal IgG (1µg) instead of DFFA Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000) Lane 2: DFFA Antibody (4µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (20µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating DFFA in Hela whole cell lysate Lane 1: Rabbit monoclonal IgG (1µg) instead of DFFA Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000) Lane 2: DFFA Antibody (4µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (20µg)

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer using antibody at 1:100 dilution.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer using antibody at 1:100 dilution.